Journal ArticleDOI
High‐resolution imaging and nano‐manipulation of biological structures on surface
TLDR
The results demonstrate that not only the high resolution capacity of the AFM is suited to resolve certain biological questions, but can also be applied to single molecule isolation and biomechanical analysis with its unique advantages.Abstract:
In this mini-review we discuss our recent findings on imaging and manipulation of biological macromolecular structures by atomic force microscopy (AFM). In the first part of this review, we focus on high-resolution imaging of selected biological samples. AFM images of membrane proteins have revealed detailed conformational features related to identifiable biological functions. Different self-assembling behaviors of short peptides into supramolecular structures on various substrates under controlled environmental conditions have been systematically studied with AFM imaging. In the second part, we present a novel nano-manipulation technique for manipulating, isolating, amplifying, and sequencing of individual DNA molecules, which may find unique applications in the analysis of difficult sequence structures. Finally, we discuss how to characterize the elasticity of individual biomolecules and live cells. These results demonstrate that not only the high resolution capacity of the AFM is suited to resolve certain biological questions, but can also be applied to single molecule isolation and biomechanical analysis with its unique advantages. Microsc. Res. Tech. 74: 614-626, 2011. (C) 2010 Wiley-Liss, Inc.read more
Citations
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Journal ArticleDOI
Scanning ion conductance microscopy for imaging biological samples in liquid: a comparative study with atomic force microscopy and scanning electron microscopy.
TL;DR: It is demonstrated that SICM imaging, especially using an ARS/hopping mode, is a useful technique with unique capabilities for imaging the three-dimensional topography of a range of biological samples under physiologically relevant aqueous conditions.
Journal ArticleDOI
Cellular interactions of doxorubicin-loaded DNA-modified halloysite nanotubes
TL;DR: To investigate the potential of DNA-wrapped HNTs (HD) as a promising drug delivery carrier, doxorubicin (DOX) is introduced as a model anticancer agent and loaded onto HD, showing sustained DOX release over two weeks without initial burst of DOX indicating delayedDOX release inside cells.
Journal ArticleDOI
Seeing is believing: atomic force microscopy imaging for nanomaterial research
Jian Zhong,Juan Yan +1 more
TL;DR: A review of atomic force microscopy (AFM) imaging techniques for nanomaterial research is presented in this article, where the advantages and disadvantages of AFM imaging techniques are discussed.
Journal ArticleDOI
Tip-induced micropatterning of silk fibroin protein using in situ solution atomic force microscopy.
TL;DR: It is demonstrated that the AFM tip, in either the contact or the tapping mode, can fabricate SF micropatterns on mica with controlled surface topography, and the deposition process requires a mechanical force-induced SF sol-gel transition followed by a transfer to the mica surface at the tip-surface contact.
Journal ArticleDOI
Nanoimaging of food proteins by atomic force microscopy. Part I: Components, imaging modes, observation ways, and research types
TL;DR: As a powerful nanotech imaging tool, AFM has many advantages for food protein research and will undoubtedly be applied to study a growing number of food proteins in the future.
References
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Ryanodine Receptor Calcium Release Channels
Michael Fill,Julio A. Copello +1 more
TL;DR: Controversies surrounding the complex Ca2+ regulation of single RyR channels receive particular attention here and a large body of information is synthesized into a focused perspective of singleRyR channel function.
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Ryanodine Receptor/Ca2+ Release Channels and Their Regulation by Endogenous Effectors
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Journal ArticleDOI
Cell-free formation of protease-resistant prion protein
David A. Kocisko,David A. Kocisko,Jon H. Come,Jon H. Come,Suzette A. Priola,Bruce Chesebro,Gregory J. Raymond,Peter T. Lansbury,Byron Caughey +8 more
TL;DR: The conversion of PrPc to protease-resistant forms similar to PrPSc in a cell-free system composed of substantially purified constituents is reported, providing direct evidence that PrP sc derives from specific PrP c–PrPSc interactions.
Journal ArticleDOI
Measuring the viscoelastic properties of human platelets with the atomic force microscope
TL;DR: The elastic modulus of the platelet was determined with a lateral resolution of approximately 100 nm by measuring force curves as a function of the lateral position on top of human platelets with the atomic force microscope.
Journal ArticleDOI
Direct measurement of the forces between complementary strands of DNA
TL;DR: Adhesive forces measured between complementary 20-base strands fell into three distinct distributions centered at 1.52, 1.11, and 0.83 nano-newtons, which are associated with the rupture of the interchain interaction between a single pair of molecules involving 20, 16, and 12 base pairs.