Journal ArticleDOI
HSP12, a new small heat shock gene of Saccharomyces cerevisiae: Analysis of structure, regulation and function
Uta Praekelt,Peter A. Meacock +1 more
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TLDR
Analysis of HSP12 expression in mutants affected in cAMP-dependent protein phosphorylation suggests that the gene is regulated by cAMP as well as heat shock, with structural similarity to other small heat shock proteins.Abstract:
We have isolated a new small heat shock gene, HSP12, from Saccharomyces cerevisiae. It encodes a polypeptide of predicted Mr 12 kDa, with structural similarity to other small heat shock proteins. HSP12 gene expression is induced several hundred-fold by heat shock and on entry into stationary phase. HSP12 mRNA is undetectable during exponential growth in rich medium, but low levels are present when cells are grown in minimal medium. Analysis of HSP12 expression in mutants affected in cAMP-dependent protein phosphorylation suggests that the gene is regulated by cAMP as well as heat shock. A disruption of the HSP12 coding region results in the loss of an abundant 14.4 kDa protein present in heat shocked and stationary phase cells. It also leads to the induction of the heat shock response under conditions normally associated with low-level HSP12 expression. The HSP12 disruption has no observable effect on growth at various temperatures, nor on the ability to acquire thermotolerance.read more
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Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
Journal ArticleDOI
The saccharomyces cerevisiae zinc finger proteins msn2p and msn4p are required for transcriptional induction through the stress-response element (stre )
María Teresa Martínez-Pastor,G Marchler,Christoph Schüller,Aron Marchler-Bauer,H Ruis,F Estruch +5 more
TL;DR: The results suggest that MSN2 and MSN4 encode a DNA‐binding component of the stress responsive system and it is likely that they act as positive transcription factors.
Journal ArticleDOI
Oxidative stress responses of the yeast Saccharomyces cerevisiae.
TL;DR: This review concentrates on the oxidant defence systems of the budding yeast Saccharomyces cerevisiae, which appears to have a number of inducible adaptive stress responses to oxidants, such as H2 O2 , superoxide anion and lipid peroxidation products.
Journal ArticleDOI
Effect of trehalose on protein structure
Nishant Kumar Jain,Ipsita Roy +1 more
TL;DR: This review aims to highlight the changing perception of the role of trehalose over the last 10 years and to propose common mechanisms that may be involved in all the myriad ways in whichtrehalose stabilizes protein structures.
Journal ArticleDOI
Stationary phase in the yeast Saccharomyces cerevisiae.
TL;DR: It is proposed that cell cycle arrest coordinated with the ability to remain viable in the absence of additional nutrients provides a good operational definition of starvation-induced stationary phase.
References
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TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
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Journal ArticleDOI
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Jack Kyte,Russell F. Doolittle +1 more
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
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