Journal ArticleDOI
Human leukocyte interferon produced by E. coli is biologically active
David V. Goeddel,Elizabeth Mcleod Yelverton,Axel Ullrich,Herbert L. Heyneker,Giuseppe Miozzari,William E. Holmes,Peter H. Seeburg,Thomas J. Dull,Laurie May,Nowell Stebbing,Roberto Crea,Shuichiro Maeda,Russell McCandliss,Alan Sloma,John M. Tabor,Mitchell Gross,Phillip C. Dr. Familletti,Sidney Pestka +17 more
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A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli, and protected squirrel monkeys from lethal encephalomyocarditis virus infection.Abstract:
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.read more
Citations
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Journal ArticleDOI
Prediction of protein antigenic determinants from amino acid sequences.
Thomas P. Hopp,Kenneth R. Woods +1 more
TL;DR: The method was developed using 12 proteins for which extensive immunochemical analysis has been carried out and subsequently was used to predict antigenic determinants for the following proteins, finding that the prediction success rate depended on averaging group length.
Journal ArticleDOI
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
TL;DR: The sequence CCAGCCAUG (G) thus emerges as a consensus sequence for eukaryotic initiation sites, and the extent to which the ribosome binding site in a given mRNA matches the -1 to -5 consensus sequence varies.
Journal ArticleDOI
Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli
Diane Pennica,William E. Holmes,William J. Kohr,Richard N. Harkins,Gordon A. Vehar,Carole A. Ward,William F. Bennett,Elizabeth Mcleod Yelverton,Peter H. Seeburg,Herbert L. Heyneker,David V. Goeddel,Desire Collen +11 more
TL;DR: Bacterial clones containing human tissue-type plasminogen activator cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells and a polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA.
Patent
Albumin Fusion Proteins
TL;DR: The present invention encompasses albumin fusion proteins as discussed by the authors, as well as vectors containing these nucleic acids, host cells transformed with the nucleic acid vectors, and methods of making the fusion proteins of the invention.
Journal ArticleDOI
Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations.
TL;DR: Recommendations for probe design and hybridization are presented, and the choice between using multiple probes reflecting all codon possibilities and a unique optimal probe is discussed.
References
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Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
TL;DR: This method can be used to isolate clones of ColE1 hybrid plasmids that contain Drosophila melanogaster genes for 18 and 28S rRNAs and any gene whose base sequence is represented in an available RNA.