Journal ArticleDOI
Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations.
TLDR
Recommendations for probe design and hybridization are presented, and the choice between using multiple probes reflecting all codon possibilities and a unique optimal probe is discussed.About:
This article is published in Journal of Molecular Biology.The article was published on 1985-05-05. It has received 844 citations till now. The article focuses on the topics: Multiplex ligation-dependent probe amplification & Genetic code.read more
Citations
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A novel potent vasoconstrictor peptide produced by vascular endothelial cells.
Masashi Yanagisawa,Hiroki Kurihara,Hiroki Kurihara,Sadao Kimura,Yoko Tomobe,Mieko Kobayashi,Youji Mitsui,Yoshio Yazaki,Katsutoshi Goto,Tomoh Masaki +9 more
TL;DR: Cloning and sequencing of preproendothelin complementary DNA shows that mature endothelin is generated through an unusual proteolytic processing, and regional homologies to a group of neurotoxins suggest that endothelins is an endogenous modulator of voltage-dependent ion channels.
Journal ArticleDOI
Vascular endothelial growth factor is a secreted angiogenic mitogen
TL;DR: DNA sequencing suggests the existence of several molecular species of VEGF, a heparin-binding growth factor specific for vascular endothelial cells that is able to induce angiogenesis in vivo.
Journal ArticleDOI
Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators
Isabelle Issemann,Stephen Green +1 more
TL;DR: A member of the steroid hormone receptor superfamily of ligand-activated transcription factors is cloned that is activated by a diverse class of rodent hepatocarcinogens that causes proliferation of peroxisomes.
Journal ArticleDOI
Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein.
Xiao Jian Sun,P. Rothenberg,P. Rothenberg,C R Kahn,Jonathan M. Backer,E. Araki,P. A. Wilden,D. A. Cahill,Barry J. Goldstein,Morris F. White +9 more
TL;DR: During insulin stimulation, the IRS-1 protein undergoes tyrosine phosphorylation and binds phosphatidylinositol 3-kinase, suggesting that IRS–1 acts as a multisite Mocking' protein to bind signal-transducing molecules containing Src-homology 2 and SRC-Homology-3 domains, which may link the insulin receptor kinase and enzymes regulating cellular growth and metabolism.
Journal ArticleDOI
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
TL;DR: It is found that purified Sp1 requires Zn(II) for sequence-specific binding to DNA, and it is likely that Sp1 interacts with DNA by binding of the Zn (II) fingers.
References
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Journal ArticleDOI
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells
Axel Ullrich,Lisa M. Coussens,Joel S. Hayflick,Thomas J. Dull,Alane M. Gray,A W Tam,Jeffrey E. Lee,Yosef Yarden,Towia A. Libermann,Joseph Schlessinger +9 more
TL;DR: The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted ν-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains.
Journal ArticleDOI
Improved Estimation of Secondary Structure in Ribonucleic Acids
Ignacio Tinoco,Philip N. Borer,Barbara Dengler,Mark D. Levin,Olke C. Uhlenbeck,Donald M. Crothers,J. Bralla +6 more
TL;DR: This method can be used for predicting and assessing possible secondary structures for recently determined RNA sequences and new experimental and theoretical results allow us to improve the method, without making it more complicated.
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Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Journal ArticleDOI
DNA methylation and the frequency of CpG in animal DNA.
TL;DR: An analysis of nearest neighbour dinucleotide frequencies and the level of DNA methylation in animals strongly supports the suggestion that 5-methylcytosine (5mC) tends to mutate abnormally frequently to T.
Journal ArticleDOI
Codon catalog usage is a genome strategy modulated for gene expressivity
TL;DR: The nucleic acid sequence bank now contains 161 mRNAs, 43 new genes are added, and internal regulation of mRNA expression by different third base choices between quartet and duet codons is proposed for bacterial genes.