Journal ArticleDOI
Human Obese Gene Expression: Adipocyte-Specific Expression and Regional Differences in the Adipose Tissue
Hiroaki Masuzaki,Yoshihiro Ogawa,Naohi Isse,Noriko Satoh,Taku Okazaki,Michika Shigemoto,Kiyoshi Mori,Naohisa Tamura,Kiminori Hosoda,Yasunao Yoshimasa,Hisato Jingami,Teruo Kawada,Kazuwa Nakao +12 more
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TLDR
In this paper, a full-length human ob complementary DNA (cDNA) clone was isolated and examined for the tissue distribution of ob gene expression in humans, which revealed that the human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins.Abstract:
The obese (ob) gene, the mutation of which results in severe hereditary obesity and diabetes in mice, has recently been isolated through positional cloning. In this study, we isolated a full-length human ob complementary DNA (cDNA) clone and examined the tissue distribution of ob gene expression in humans. The nucleotide sequences of the human ob cDNA coding region were 83% identical to those of the mouse and rat ob cDNA coding regions. Analysis of the deduced amino acid sequences revealed that the human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis using the cloned human ob cDNA fragment as a probe identified a single messenger RNA (mRNA) species 4.5 kb in size found abundantly in the adipose tissues obtained from the subcutaneous, omental, retroperitoneal, perilymphatic, and mesenteric fat pads. However, no significant amount of ob mRNA was present in the brain, heart, lung, liver, stomach, pancreas, spleen, small intestine, kidney, prostate, testis, colon, or skeletal muscle. The ob mRNA level in the adipose tissue varied from region to region even in the same individual. Furthermore, in the human adipose tissue, ob gene expression occurred in mature adipocytes rather than in stromal-vascular cells. This study is the first report of the elucidation of ob gene expression in human tissues, thereby leading to better understanding of the physiological and clinical implications of the ob gene.read more
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Leptin levels in human and rodent : Measurement of plasma leptin and ob RNA in obese and weight-reduced subjects
Margherita Maffei,Jeffrey L. Halaas,Eric Ravussin,Richard E. Pratley,Gwo-Hwa Lee,Gwo-Hwa Lee,Yan Zhang,Yan Zhang,Hong Fei,S. Kim,R. Lallone,S. Ranganathan,Philip A. Kern,Philip A. Kern,Jeffrey M. Friedman,Jeffrey M. Friedman +15 more
TL;DR: Weight loss due to food restriction was associated with a decrease in plasma leptin in samples from mice and obese humans, suggesting differences in its secretion rate from fat.
Journal ArticleDOI
Understanding Adipocyte Differentiation
TL;DR: Characterization of regulatory regions of adipose-specific genes has led to the identification of the transcription factors peroxisome proliferator-activated receptor-gamma and CCAAT/enhancer binding protein (C/EBP), which play a key role in the complex transcriptional cascade during adipocyte differentiation.
Journal ArticleDOI
Nonadipose tissue production of leptin: leptin as a novel placenta-derived hormone in humans.
Hiroaki Masuzaki,Yoshihiro Ogawa,Norimasa Sagawa,Kiminori Hosoda,T. Matsumoto,Hiroko Mise,Haruo Nishimura,Yasunao Yoshimasa,Issei Tanaka,T. Mori,Kazuwa Nakao +10 more
TL;DR: Evidence is provided for leptin as a novel placenta-derived hormone in humans and the physiologic and pathophysiologic significance of leptin in normal pregnancy and gestational trophoblastic neoplasms is suggested.
Journal ArticleDOI
The role of leptin and ghrelin in the regulation of food intake and body weight in humans: a review.
TL;DR: A review of the role of leptin and ghrelin in food intake and body weight in humans and their mechanism of action is presented in this article, where possible abnormalities in the leptin and Ghrelin systems that may contribute to the development of obesity are discussed.
Journal ArticleDOI
Transient increase in obese gene expression after food intake or insulin administration.
Régis Saladin,P De Vos,Michèle Guerre-Millo,A Leturque,Jean-Philippe Girard,Bart Staels,Johan Auwerx +6 more
TL;DR: It is shown that ob gene exhibits diurnal variation, increasing during the night, after rats start eating, which is linked to changes in food intake, as fasting prevented the cyclic variation and decreased ob messenger RNA.