Journal ArticleDOI
Weight-Reducing Effects of the Plasma Protein Encoded by the obese Gene
Jeffrey L. Halaas,Gajiwala Ketan,Margherita Maffei,Steven L. Cohen,Brian T. Chait,Daniel Rabinowitz,Roger Lallone,Stephen K. Burley,Jeffrey M. Friedman +8 more
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TLDR
Injection of wild-type mice twice daily with the mouse protein resulted in a sustained 12 percent weight loss, decreased food intake, and a reduction of body fat from 12.2 to 0.7 percent, suggesting that the OB protein serves an endocrine function to regulate body fat stores.Abstract:
The gene product of the ob locus is important in the regulation of body weight. The ob product was shown to be present as a 16-kilodalton protein in mouse and human plasma but was undetectable in plasma from C57BL/6J ob/ob mice. Plasma levels of this protein were increased in diabetic (db) mice, a mutant thought to be resistant to the effects of ob. Daily intraperitoneal injections of either mouse or human recombinant OB protein reduced the body weight of ob/ob mice by 30 percent after 2 weeks of treatment with no apparent toxicity but had no effect on db/db mice. The protein reduced food intake and increased energy expenditure in ob/ob mice. Injections of wild-type mice twice daily with the mouse protein resulted in a sustained 12 percent weight loss, decreased food intake, and a reduction of body fat from 12.2 to 0.7 percent. These data suggest that the OB protein serves an endocrine function to regulate body fat stores.read more
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Journal ArticleDOI
Serum Immunoreactive-Leptin Concentrations in Normal-Weight and Obese Humans
Robert V. Considine,Madhur K. Sinha,Mark L. Heiman,Aidas Kriauciunas,Thomas W. Stephens,Mark R. Nyce,Joanna P. Ohannesian,Cheryl C. Marco,Linda J. McKee,Thomas L. Bauer,Jose F. Caro +10 more
TL;DR: Serum leptin concentrations are correlated with the percentage of body fat, suggesting that most obese persons are insensitive to endogenous leptin production.
Journal ArticleDOI
Leptin and the regulation of body weight in mammals
TL;DR: The role of leptin in the control of body weight and its relevance to the pathogenesis of obesity are reviewed.
Journal ArticleDOI
Insulin signalling and the regulation of glucose and lipid metabolism
Alan R. Saltiel,C. Ronald Kahn +1 more
TL;DR: The epidemic of type 2 diabetes and impaired glucose tolerance is one of the main causes of morbidity and mortality worldwide, and tissues such as muscle, fat and liver become less responsive or resistant to insulin.
Journal ArticleDOI
Leptin levels in human and rodent : Measurement of plasma leptin and ob RNA in obese and weight-reduced subjects
Margherita Maffei,Jeffrey L. Halaas,Eric Ravussin,Richard E. Pratley,Gwo-Hwa Lee,Gwo-Hwa Lee,Yan Zhang,Yan Zhang,Hong Fei,S. Kim,R. Lallone,S. Ranganathan,Philip A. Kern,Philip A. Kern,Jeffrey M. Friedman,Jeffrey M. Friedman +15 more
TL;DR: Weight loss due to food restriction was associated with a decrease in plasma leptin in samples from mice and obese humans, suggesting differences in its secretion rate from fat.
Journal ArticleDOI
Identification and expression cloning of a leptin receptor Ob-R
Louis A. Tartaglia,Marlene Dembski,Xun Weng,Nanhua Deng,Janice A. Culpepper,Rene Devos,Grayson J. Richards,L. Arthur Campfield,Frederick T. Clark,Jim Deeds,Craig Muir,Sean Sanker,Ann Moriarty,Karen J. Moore,John S. Smutko,Gail G. Mays,Elizabeth A. Wool,Cheryl A. Monroe,Robert I. Tepper +18 more
TL;DR: The ob gene product, leptin, is an important circulating signal for the regulation of body weight and a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [125I]leptin fusion proteins were generated to identify high affinity leptin-binding sites in the mouse choroid plexus.
References
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Book
Antibodies: A Laboratory Manual
Ed Harlow,David P. Lane +1 more
TL;DR: A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.
Journal ArticleDOI
Positional cloning of the mouse obese gene and its human homologue
Yiying Zhang,Ricardo Proenca,Ricardo Proenca,Margherita Maffei,Marisa Barone,Marisa Barone,Lori Leopold,Lori Leopold,Jeffrey M. Friedman,Jeffrey M. Friedman +9 more
TL;DR: The ob gene product may function as part of a signalling pathway from adipose tissue that acts to regulate the size of the body fat depot.
Book ChapterDOI
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Journal ArticleDOI
Changes in Energy Expenditure Resulting from Altered Body Weight
TL;DR: Maintenance of a reduced or elevated body weight is associated with compensatory changes in energy expenditure, which oppose the maintenance of a body weight that is different from the usual weight, which may account for the poor long-term efficacy of treatments for obesity.
Journal ArticleDOI
The role of depot fat in the hypothalamic control of food intake in the rat.
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