Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells
Girolama La Mantia,Domenico Maglione,Gina Pengue,Antonio Di Cristofano,Antonio Simeone,Luisa Lanfrancone,Luigi Lania +6 more
TLDR
It is shown that ERV-9 LTR sequences are capable to drive expression of linked CAT gene in a cell specific manner as LTR promoter activity has been detected only in NT2/D1 cells.Abstract:
A novel endogenous retroviral sequence (ERV-9) has been isolated from a human embryonal carcinoma cDNA library by hybridization to a probe containing a recently described human repetitive element. DNA sequence analysis of the 4kb cDNA insert (pHE.1) revealed the presence of ORFs potentially coding for putative retrovirus-related gag, pol and env proteins. Northern blot and RNase protection experiments showed that RNA homologous to the pHE.1 insert is detected only in embryonal carcinoma cells as a 8 kb mRNA, and its expression is negatively regulated during retinoic acid induced differentiation of the human teratocarcinoma cell line NT2/D1. Using a pol specific probe we have isolated a genomic locus containing the ERV-9 sequences. Characterization by restriction enzyme analysis and DNA sequencing allowed us to define LTR-like sequences, that are composed by a complex array of subrepetitive elements. In addition we show that ERV-9 LTR sequences are capable to drive expression of linked CAT gene in a cell specific manner as LTR promoter activity has been detected only in NT2/D1 cells.read more
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The viruses in all of us: characteristics and biological significance of human endogenous retrovirus sequences
TL;DR: The demonstrable and potential roles of HTDV/HERV-K as well as of other human elements in disease and in maintaining genome plasticity are illustrated.
Journal ArticleDOI
Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis
Hervé Perron,Jeremy A. Garson,Frederic Bedin,Frederic Beseme,Gláucia Paranhos-Baccalà,Florence Komurian-Pradel,François Mallet,Philip W. Tuke,Cécile Voisset,J.-L. Blond,B. Lalande,J M Seigneurin,Bernard Mandrand +12 more
TL;DR: Whether MSRV represents an exogenous retrovirus with closely related endogenous elements or a replication-competent, virion-producing, endogenous provirus is as yet unknown, and further molecular epidemiological studies are required to determine precisely the apparent association of virions containing MSRv RNA with MS.
Journal ArticleDOI
Molecular Characterization and Placental Expression of HERV-W, a New Human Endogenous Retrovirus Family
Jean-Luc Blond,Frederic Beseme,Laurent Duret,Olivier Bouton,Frederic Bedin,Hervé Perron,Bernard Mandrand,François Mallet +7 more
TL;DR: The multiple sclerosis-associated retrovirus isolated from plasma of MS patients was found to be phylogenetically and experimentally related to human endogenous retroviruses (HERVs), and a new HERV family was named HERV-W.
Journal ArticleDOI
Retroviral RNA identified in the cerebrospinal fluids and brains of individuals with schizophrenia
Håkan Karlsson,Håkan Karlsson,Silke Bachmann,Johannes Schröder,Justin C. McArthur,E. Fuller Torrey,Robert H. Yolken +6 more
TL;DR: The transcriptional activation of certain retroviral elements within the central nervous system of individuals with schizophrenia may be associated with the development of schizophrenia in at least some individuals, and the further characterization of retrov Viral pol genes might lead to improved methods for the diagnosis and management of this disorder.
Journal ArticleDOI
Identification and Characterization of Novel Human Endogenous Retrovirus Families by Phylogenetic Screening of the Human Genome Mapping Project Database
TL;DR: Members of each of the 10 families are defective, and calculation of their integration dates suggested that most of them are likely to have been present within the human lineage since it diverged from the Old World monkeys more than 25 million years ago.
References
More filters
Journal ArticleDOI
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
TL;DR: A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.
Journal ArticleDOI
Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.
TL;DR: The provirus of ATLV integrated in DNA of leukemia T cells from a patient with ATL was molecularly cloned and the complete nucleotide sequence of the proviral genome was determined.
Journal ArticleDOI
Nucleotide sequence of the AIDS virus, LAV
TL;DR: The complete 9193-nucleotide sequence of the probable causative agent of AIDS, lymphadenopathy-associated virus (LAV), has been determined and shows two novel open reading frames the authors call Q and F, which place LAV apart from the previously characterized family of human T cell leukemia/lymphoma viruses.
Journal ArticleDOI
Identification of four conserved motifs among the RNA-dependent polymerase encoding elements.
TL;DR: At the evolutionary level, the sequence similarities, gap distribution and distances between each motif strongly suggest that the ancestral polymerase module was encoded by an individual genetic element which was most closely related to the plus‐strand RNA viruses and the non‐viral retroposons.
Journal ArticleDOI
A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo.
Mitchell H. Rosner,M A Viganò,Keiko Ozato,P. M. Timmons,F. Poirier,F. Poirier,Peter W. J. Rigby,Louis M. Staudt +7 more
TL;DR: The murine oct-3 gene encodes a transcription factor containing a POU-specific domain and a homeodomain, which is expressed in the totipotent and pluripotent stem cells of the pre-gastrulation embryo and is down-regulated during differentiation to endoderm and mesoderm, suggesting that it has a role in early development.