Identification of a 47 kDa fibronectin‐binding protein expressed by Borrelia burgdorferi isolate B31
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TLDR
The identification of BBK32 as a receptor for fibronectin binding may enhance the understanding of the pathogenesis and chronic nature of Lyme disease.Abstract:
The attachment of pathogenic microorganisms to host cells and tissues is often mediated through the expression of surface receptors recognizing components of the extracellular matrix (ECM). Here, we investigate the ability of Borrelia spirochaetes to bind the ECM constituent, fibronectin. Borrelia lysates were separated by SDS-PAGE, transferred to nitrocellulose and probed with alkaline phosphatase-labelled fibronectin (fibronectin-AP). Five of six Borrelia species and four of eight B. burgdorferi sensu lato isolates expressed one or more fibronectin-binding proteins. Borrelia burgdorferi isolate B31 expressed a 47 kDa (P47) fibronectin-binding protein that was localized to the outer envelope based on susceptibility to proteinase K. The interaction of P47 with fibronectin was specific, and the region of fibronectin bound by P47 mapped to the gelatin/collagen binding domain. P47 was purified by affinity chromatography, digested with endoproteinase Lys-C, and the peptide fragments analysed by liquid chromatography/tandem mass spectroscopy. A search of protein databases disclosed that the P47 peptide mass profile matched that predicted for the bbk32 gene product of B. burgdorferi isolate B31. The bbk32 gene was cloned into Escherichia coli, and the ability of recombinant BBK32 to bind fibronectin and inhibit the attachment of B. burgdorferi was demonstrated. The identification of BBK32 as a receptor for fibronectin binding may enhance our understanding of the pathogenesis and chronic nature of Lyme disease.read more
Citations
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A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi.
Sherwood R. Casjens,Nanette Palmer,René Van Vugt,Wai Mun Huang,Brian Stevenson,Patricia A. Rosa,Raju Lathigra,Granger G. Sutton,Jeremy Peterson,Robert J. Dodson,Daniel H. Haft,Erin Hickey,Michelle L. Gwinn,Owen White,Claire M. Fraser +14 more
TL;DR: It is determined that Borrelia burgdorferi strain B31 MI carries 21 extrachromosomal DNA elements, the largest number known for any bacterium, and the nucleotide sequence of three linear and seven circular plasmids in this infectious isolate is reported.
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The complement regulator factor H binds to the surface protein OspE of Borrelia burgdorferi.
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References
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Journal ArticleDOI
Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi
Claire M. Fraser,Sherwood R. Casjens,Wai Mun Huang,Granger G. Sutton,Rebecca A. Clayton,Raju Lathigra,Owen White,Karen A. Ketchum,Robert J. Dodson,Erin Hickey,Michelle L. Gwinn,Brian Dougherty,J F Tomb,Robert D. Fleischmann,Delwood Richardson,Jeremy Peterson,Anthony R. Kerlavage,John Quackenbush,Steven L. Salzberg,Mark S. Hanson,René Van Vugt,Nanette Palmer,Mark Raymond Adams,Jeannine D. Gocayne,Janice Weidman,Teresa Utterback,Larry Watthey,Lisa McDonald,Patricia Artiach,Cheryl Bowman,Stacey Garland,Claire Fujii,Matthew D. Cotton,Kurt Horst,Kevin Roberts,Bonnie Hatch,Hamilton O. Smith,J. Craig Venter +37 more
TL;DR: The genome of the bacterium Borrelia burgdorferi B31, the aetiologic agent of Lyme disease, contains a linear chromosome of 910,725 base pairs and at least 17 linear and circular plasmids with a combined size of more than 533,000 base pairs, which suggest their limited metabolic capacities reflect convergent evolution by gene loss from more metabolically competent progenitors.
Journal ArticleDOI
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TL;DR: MSCRAMMs recognizing fibronectin-, fibrinogen-, collagen-, and heparin-related polysaccharides are discussed in terms of structural organization, ligand-binding structures, importance in host tissue colonization and invasion, and role as virulence factors.
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Medical progress. Lyme disease
TL;DR: Mise a jour des connaissances sur la maladie, due a Borrelia burgdorferi, transmise par tiques, role des souris dans la survie de B. b.
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TL;DR: Three DNA relatedness groups were associated with specific rRNA gene restriction patterns, protein electrophoresis patterns, and patterns of reactivity with murine monoclonal antibodies in Borrelia isolates associated with Lyme borreliosis.
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