Identification of canola roots endophytic bacteria and analysis of their potential as biofertilizers for canola crops with special emphasis on sporulating bacteria
Pilar Martínez-Hidalgo,José David Flores-Félix,Fernando Sánchez-Juanes,Raúl Rivas,Pedro F. Mateos,Ignacio Santa Regina,Alvaro Peix,Eustoquio Martínez-Molina,José Mariano Igual,Encarna Velázquez +9 more
TLDR
In this work, a collection of bacterial endophytes isolated from canola roots were identified using MALDI-TOF MS, a technique that is still rarely used for the identification of such bacteria, and rrs gene sequencing, a methodology that is commonly used to identify canolaendophytes.Abstract:
Canola (Brassica napus L. var. oleracea) is the third most common oil-producing crop worldwide after palm and soybean. Canola cultivation requires the use of chemical fertilizers, but the amount required can be reduced by applying plant growth-promoting bacteria (PGPB). Among PGPB, endophytic bacteria have certain advantages as biofertilizers, but canola endophytic bacteria have rarely been studied. In this work, we identified a collection of bacterial endophytes isolated from canola roots using MALDI-TOF MS, a technique that is still rarely used for the identification of such bacteria, and rrs gene sequencing, a methodology that is commonly used to identify canola endophytes. The results demonstrated that some bacterial isolates from canola roots belonged to the genera Bacillus, Neobacillus, Peribacillus (Pe.), and Terribacillus, but most isolates belonged to the genera Paenibacillus (P.) and Pseudomonas (Ps.). Inoculation of these isolates indicated that several of them could efficiently promote canola seedling growth in hydroponic conditions. These results were then confirmed in a microcosm experiment using agricultural soil, which demonstrated that several isolates of Pseudomonas thivervalensis, Paenibacillus amylolyticus, Paenibacillus polymyxa, Paenibacillus sp. (Paenibacillus glucanolyticus/Paenibacillus lautus group), and Peribacillus simplex (previously Bacillus simplex) could efficiently promote canola shoot growth under greenhouse conditions. Among them, the isolates of Paenibacillus and Peribacillus were the most promising biofertilizers for canola crops as they are sporulated rods, which is an advantageous trait when formulating biofertilizers.read more
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Strategies to Enhance the Use of Endophytes as Bioinoculants in Agriculture
Bartholomew Saanu Adeleke,Ayomide Emmanuel Fadiji,Modupe Stella Ayilara,Ozede N. Igiehon,Blessing Chidinma Nwachukwu,Olubukola Oluranti Babalola +5 more
TL;DR: The need to unravel microbial strategies for yield enhancement and pathogen suppressiveness have become imperative in agricultural biotechnology and this review focuses on the endophytic microbiome, mechanisms, factors influencing endophyte recruitment, and strategies for possible exploration as bioinoculants.
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Novel Approaches for Sustainable Horticultural Crop Production: Advances and Prospects
Oksana Lastochkina,Sasan Aliniaeifard,Maryam Seifikalhor,Massimo H. Bosacchi,D. R. Maslennikova,A. R. Lubyanova +5 more
TL;DR: In this paper, the authors highlight the prospective impacts that are positive challenges for the application of beneficial microbial endophytes, nanomaterials (NMs), exogenous phytohormones strigolactones (SLs), as well as controlled environment horticulture (CEH) using artificial light in sustainable production of horticultural crops.
Journal ArticleDOI
The Antibacterial, Antitumor Activities, and Bioactive Constituents’ Identification of Alectra sessiliflora Bacterial Endophytes
TL;DR: For the first time, bacterial endophytes associated with A. sessiliflora are reported with antibacterial and antitumor activities with reported biological activity, such as antimicrobial, antioxidant, anti-inflammatory, antitumors, and antidiabetic properties.
Journal ArticleDOI
Bioprospecting and Challenges of Plant Microbiome Research for Sustainable Agriculture, a Review on Soybean Endophytic Bacteria
TL;DR: In this paper , a review of soybean endophytic bacteria and their potential use in sustainable agriculture is presented, where the use of molecular techniques in unraveling the functions of soybeans is explored.
References
More filters
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
Plant growth-promoting bacterial endophytes.
TL;DR: Genome comparisons between bacterial endophytes and the genomes of rhizospheric plant growth-promoting bacteria are starting to unveil potential genetic factors involved in an endophytic lifestyle, which should facilitate a better understanding of the functioning of bacterialendophytes.
Journal ArticleDOI
Microbial culturomics: paradigm shift in the human gut microbiome study
Jean-Christophe Lagier,Fabrice Armougom,Matthieu Million,Perrine Hugon,Isabelle Pagnier,Catherine Robert,Fadi Bittar,Ghislain Fournous,Gregory Gimenez,Marie Maraninchi,Jean-François Trape,Eugene V. Koonin,B. La Scola,Didier Raoult +13 more
TL;DR: Culturomics complements metagenomics by overcoming the depth bias inherent in metagenomic approaches, and identifies 174 species never described previously in the human gut.
Journal ArticleDOI
A review on the plant microbiome: Ecology, functions, and emerging trends in microbial application
TL;DR: In this paper, the importance and functionalities of the bacterial plant microbiome and discusses challenges and concepts in regard to the application of plantassociated bacteria. But, the authors do not consider the impact of farming practices and genotype on the microbial community.
Journal ArticleDOI
Culturing the human microbiota and culturomics
Jean-Christophe Lagier,Grégory Dubourg,Matthieu Million,Frédéric Cadoret,Melhem Bilen,Florence Fenollar,Anthony Levasseur,Jean-Marc Rolain,Pierre-Edouard Fournier,Didier Raoult +9 more
TL;DR: How culturomics has extended the understanding of bacterial diversity, and how it can be applied to the study of the human microbiota and the potential implications for human health are described.