Imaging proteins at the single-molecule level
Jean-Nicolas Longchamp,Stephan Rauschenbach,Sabine Abb,Conrad Escher,Tatiana Latychevskaia,Klaus Kern,Klaus Kern,Hans-Werner Fink +7 more
TLDR
The method to image and reveal structural details of proteins on a truly single-molecule level is reported, using low-energy electron holography to image individual proteins electrospray deposited on freestanding graphene.Abstract:
Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by low-energy electron holography. Samples of individual proteins and protein complexes on ultraclean freestanding graphene were prepared by soft-landing electrospray ion beam deposition, which allows chemical- and conformational-specific selection and gentle deposition. Low-energy electrons do not induce radiation damage, which enables acquiring subnanometer resolution images of individual proteins (cytochrome C and BSA) as well as of protein complexes (hemoglobin), which are not the result of an averaging process.read more
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Hierarchy of Hybrid Materials-The Place of Inorganics-in-Organics in it, Their Composition and Applications.
Mariia Saveleva,Mariia Saveleva,Karaneh Eftekhari,Anatoly Abalymov,Timothy E.L. Douglas,Dmitry Volodkin,Bogdan Parakhonskiy,Andre G. Skirtach +7 more
TL;DR: This work provides here a general classification of hybrid materials, wherein organics–in-inorganics (inorganic materials modified by organic moieties) are distinguished from inorganics– in–organic (organic materials or matrices modified by inorganic constituents).
Journal ArticleDOI
Strong-field nano-optics
Péter Dombi,Zsuzsanna Pápa,Jan Vogelsang,Sergey V. Yalunin,Murat Sivis,Georg Herink,Sascha Schäfer,Petra Groß,Claus Ropers,Christoph Lienau +9 more
TL;DR: In the strong-field regime, the amplitude and phase of the external electromagnetic field can exceed or exceed the field strengths that bind the electrons inside the medium as mentioned in this paper. But this is not the case in the non-perturbative regime.
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Attosecond physics phenomena at nanometric tips
Michael Krüger,Christoph Lemell,Georg Wachter,Joachim Burgdörfer,Peter Hommelhoff,Peter Hommelhoff +5 more
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Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry.
Mowei Zhou,Carter Lantz,Kyle A. Brown,Ying Ge,Ljiljana Paša-Tolić,Joseph A. Loo,Frederik Lermyte +6 more
TL;DR: While this work is written primarily from a mass spectrometry perspective, it is targeted to all bioanalytical scientists who are interested in applying these methods to their own biochemistry and chemical biology research.
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The numerics of phase retrieval
Albert Fannjiang,Thomas Strohmer +1 more
TL;DR: The past decade has witnessed a surge in the systematic study of computational algorithms for phase retrieval, and this paper will review recent advances from a numerical viewpoint.
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Journal ArticleDOI
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TL;DR: This work offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage, by using pulses briefer than the timescale of most damage processes.