Immune responses induced by the Leishmania (Leishmania) donovani A2 antigen, but not by the LACK antigen, are protective against experimental Leishmania (Leishmania) amazonensis infection
Eduardo A.F. Coelho,Carlos Alberto Pereira Tavares,Fernando A. A. Carvalho,Karina Figueiredo Chaves,Kadima Nayara Teixeira,Rafaela Chitarra Rodrigues,Hugues Charest,Greg Matlashewski,Ricardo T. Gazzinelli,Ana Paula Fernandes +9 more
TLDR
A2, but not LACK, fits the requirements for a safe vaccine against American leishmaniasis, and the association between rA2 and rLACK antigens in the same vaccine completely inhibited the rA 2-specific IFN-γ and humoral responses and, consequently, the protective effect of the r a2 antigen against L. amazonensis infection.Abstract:
Leishmania amazonensis is one of the major etiologic agents of a broad spectrum of clinical forms of leishmaniasis and has a wide geographical distribution in the Americas, which overlaps with the areas of transmission of many other Leishmania species. The LACK and A2 antigens are shared by various Leishmania species. A2 was previously shown to induce a potent Th1 immune response and protection against L. donovani infection in BALB/c mice. LACK is effective against L. major infection, but no significant protection against L. donovani infection was observed, in spite of the induction of a potent Th1 immune response. In an attempt to select candidate antigens for an American leishmaniasis vaccine, we investigated the protective effect of these recombinant antigens (rLACK and rA2) and recombinant interleukin-12 (rIL-12) against L. amazonensis infection in BALB/c mice. As expected, immunization with either rA2-rIL-12 or rLACK-rIL-12 induced a robust Th1 response prior to infection. However, only the BALB/c mice immunized with rA2-rIL-12 were protected against infection. Sustained gamma interferon (IFN-γ) production, high levels of anti-A2 antibodies, and low levels of parasite-specific antibodies were detected in these mice after infection. In contrast, mice immunized with rLACK-rIL-12 displayed decreased levels of IFN-γ and high levels of both anti-LACK and parasite-specific antibodies. Curiously, the association between rA2 and rLACK antigens in the same vaccine completely inhibited the rA2-specific IFN-γ and humoral responses and, consequently, the protective effect of the rA2 antigen against L. amazonensis infection. We concluded that A2, but not LACK, fits the requirements for a safe vaccine against American leishmaniasis.read more
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Does the Leishmania major paradigm of pathogenesis and protection hold for New World cutaneous leishmaniases or the visceral disease
TL;DR: Different virulence factors have been identified for distinct Leishmania species, and there are profound differences in the immune mechanisms that mediate susceptibility/resistance to infection and in the pathology associated with disease.
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Protective immunity against challenge with Leishmania (Leishmania) chagasi in beagle dogs vaccinated with recombinant A2 protein.
Ana Paula Fernandes,Miriam Maria Silva Costa,Eduardo A.F. Coelho,Marilene Suzan Marques Michalick,Eloísa de Freitas,Maria Norma Melo,Wagner Luiz Tafuri,Daniela de Melo Resende,Vinícius Hermont,Christiane de Freitas Abrantes,Ricardo T. Gazzinelli,Ricardo T. Gazzinelli,Ricardo T. Gazzinelli +12 more
TL;DR: Investigation in dogs of the immunogenicity and protective immunity against Leishmania (Leishmania) chagasi infection induced by vaccination with a formulation containing the recombinant A2 protein, an amastigote specific antigen, and saponin found it necessary to allow serological differentiation between vaccinated and infected animals.
Journal ArticleDOI
Vaccine candidates for leishmaniasis: a review.
Rajeev Nagill,Sukhbir Kaur +1 more
TL;DR: Control of reservoir host and vector is difficult due to operational difficulties and frequent relapses in the host, therefore, the development of effective and affordable vaccine against leishmaniasis is highly desirable.
Journal ArticleDOI
Recombinant Leishmania tarentolae expressing the A2 virulence gene as a novel candidate vaccine against visceral leishmaniasis.
Amir Mizbani,Tahereh Taheri,Farnaz Zahedifard,Yasaman Taslimi,Hiva Azizi,Kayhan Azadmanesh,Barbara Papadopoulou,Sima Rafati +7 more
TL;DR: The results show that a single intraperitoneal administration of the A2-recombinant L. tarentolae strain protects BALB/c mice against L. infantum challenge and that protective immunity is associated with high levels of IFN-gamma production prior and after challenge.
Journal ArticleDOI
Antigenicity and Protective Efficacy of a Leishmania Amastigote-specific Protein, Member of the Super-oxygenase Family, against Visceral Leishmaniasis
Vívian T. Martins,Miguel A. Chávez-Fumagalli,Lourena E. Costa,Adriana Monte Cassiano Canavaci Martins,Paula S. Lage,Daniela P. Lage,Mariana C. Duarte,Diogo G. Valadares,Rubens Daniel Miserani Magalhães,Tatiana G. Ribeiro,Ronaldo Alves Pinto Nagem,Wanderson D. DaRocha,Wiliam César Bento Régis,Manuel Soto,Eduardo A.F. Coelho,Ana Paula Fernandes,Carlos Alberto Pereira Tavares +16 more
TL;DR: It is shown that this Leishmania oxygenase amastigote-specific protein can be used for a more sensitive and specific serodiagnosis of asymptomatic and symptomatic CVL and, when combined with a Th1-type adjuvant, can also be employ as a candidate antigen to develop vaccines against VL.
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Vaccination with dna encoding the immunodominant lack parasite antigen confers protective immunity to mice infected with leishmania major
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TL;DR: The ability of mice to control infection at sites distant to the site of vaccination suggests that systemic protection was achieved by LACK DNA vaccination, and DNA immunization may offer an attractive alternative vaccination strategy against intracellular pathogens, as compared with conventional vaccination with antigens combined with adjuvants.
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