Journal ArticleDOI
Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope protein
Kanakatte Raviprakash,Tadeusz J. Kochel,Daniel Ewing,Monika Simmons,Irving Phillips,Curtis G. Hayes,Kevin R. Porter +6 more
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Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E.About:
This article is published in Vaccine.The article was published on 2000-05-01. It has received 114 citations till now. The article focuses on the topics: Immunogenicity & DNA vaccination.read more
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West Nile Virus Recombinant DNA Vaccine Protects Mouse and Horse from Virus Challenge and Expresses In Vitro a Noninfectious Recombinant Antigen That Can Be Used in Enzyme-Linked Immunosorbent Assays
Brent S. Davis,Gwong-Jen J. Chang,Bruce C. Cropp,John T. Roehrig,Denise A. Martin,Carl J. Mitchell,Richard A. Bowen,Michel L. Bunning +7 more
TL;DR: This recombinant antigen has great potential to become the antigen of choice and will facilitate the standardization of reagents and implementation of WN virus surveillance in the United States and elsewhere.
Journal ArticleDOI
Immune Response to Dengue Virus and Prospects for a Vaccine
TL;DR: Vaccines will need to provide long-term protection against each of the four DENV serotypes by inducing neutralizing antibodies, and live, attenuated and various nonliving virus vaccines are in development.
Journal ArticleDOI
Characterization of neutralizing antibodies to West Nile virus
Melissa D. Sánchez,Theodore C. Pierson,Douglas McAllister,Sheri L. Hanna,Bridget A. Puffer,Laura E. Valentine,Mariam Murtadha,James A. Hoxie,Robert W. Doms +8 more
TL;DR: Interestingly, some non-neutralizing antibodies directed against the West Nile virus E glycoprotein bound with high affinity to domains I or III, indicating that both affinity and the precise epitope recognized by an antibody are important determinants of WNV neutralization.
Journal ArticleDOI
The development of recombinant subunit envelope-based vaccines to protect against dengue virus induced disease.
TL;DR: The DEN-80E proteins are potent immunogens when formulated with a variety of adjuvants, inducing high titer virus neutralizing antibody responses and demonstrating protection in both mouse and non-human primate models.
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Dengue type 2 virus subviral extracellular particles produced by a stably transfected mammalian cell line and their evaluation for a subunit vaccine.
Eiji Konishi,Atsuko Fujii +1 more
TL;DR: Results indicate that D cell EPs are a promising non-infectious vaccine antigen for dengue.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Flavivirus Genome Organization, Expression, and Replication
Journal ArticleDOI
Pathogenesis of dengue: challenges to molecular biology
TL;DR: This work has identified a severe syndrome, dengue hemorrhagic fever/dengue shock syndrome, in Southeast Asian children, which recently has also been identified in children infected with the virus in Puerto Rico.