Journal ArticleDOI
Increased expression of laminin receptors during myeloid differentiation
Miriam Hatsue Honda Federico,Durvanei Augusto Maria,Shigueko Sonohara,M Yamamoto,M. L.H. Katayama,Maria Mitzi Brentani +5 more
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TLDR
In this paper, 125I-laminin binding to lymphoid or myeloid leukemic cells was mainly inhibited by P1 fragments, whereas granulocytes and differentiated HL60 cells displayed a dual binding pattern for laminin fragments P1 and E8.Abstract:
Variation of laminin receptor levels (LNR) during myeloid-cell differentiation and in acute leukemia were investigated by 125I-laminin-binding determination during HL60 cell differentiation and in cells of patients with different types of leukemia, characterized according to the FAB classification. LNR levels in HL60 cells increased during differentiation, being significantly higher in cells exposed to phorbol myristate acetate (PMA) and ethanol (55,391 +/- 27,845 and 29,314 +/- 6,435 sites/cell respectively) as compared with HL60 controls (8,549 +/- 4,000 sites/cell). The control cells do not adhere to laminin-coated surfaces, but differentiation with PMA results in their rapid adherence on this substratum. Short treatment with PMA does not increase the number of adherent cells or the receptor expression. Granulocytes also presented equally high LNR concentration (29,739 +/- 13,516 sites/cell). The lymphoid cells (lymphocyte, acute lymphoid leukemia and chronic lymphocytic leukemia) shared low LNR numbers (less than 6,500 sites/cell). Myeloid cells displayed a wide range of LN receptors with higher levels being associated with the more differentiated FAB subgroups. 125I-laminin binding to lymphoid or myeloid leukemic cells was mainly inhibited by P1 fragments, whereas granulocytes and differentiated HL60 cells displayed a dual binding pattern for laminin fragments P1 and E8. These results were confirmed by assays using 125I-labelled P1 and E8 fragments. We conclude that magnitude of LNR levels and variation in expression of P1 and E8 receptors appear to be linked to lineage and maturation status in hematopoietic cells.read more
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Journal Article
Expression of the 67-kDa laminin receptor in acute myeloid leukemia cells mediates adhesion to laminin and is frequently associated with monocytic differentiation.
Nunzia Montuori,Carmine Selleri,Am Risitano,Anna Maria Raiola,Pia Ragno,L Del Vecchio,Bruno Rotoli,Guido Rossi +7 more
TL;DR: Results indicate that 67LR expression mediates specific adhesion to laminin and that the detection of this molecule may be a valuable addition to other lineage-associated antigens in identifying monocytic-oriented AML.
Journal ArticleDOI
Eosinophil Accumulation in Rat Uterus Following Estradiol Administration is Modulated by Laminin and its Integrin Receptors
Maria Lucia Hirata Katayama,Miriam Hatsue Honda Federico,Ricardo R. Brentani,Maria Mitzi Brentani +3 more
TL;DR: The results suggest that integrin-laminin interactions may contribute to the preferential eosinophil recruitment in vivo.
Journal ArticleDOI
Expression of cell kinetics and death during monocyte–macrophage differentiation: effects of Actinomycin D and Vinblastine treatments
TL;DR: The different effects of two cytostatic drugs, Actinomycin D and Vinblastine, during macrophage-like differentiation induced in THP-1 monocytic cell line byphorbol ester phorbol 12-myristate 13-acetate (PMA) were studied by morpho-cytochemical approaches.
Characterization of Hematopoietic Transcription Factor Complexes in Erythroid Cells
TL;DR: BirA-mediated tagging is an efficient approach for the direct capture and characterization of transcription factors and chromatin remodeling co-factors involved in activation and repression of gene targets.
Posted ContentDOI
Identification of biological mechanisms by semantic classifier systems
TL;DR: A semantic approach to the issue is devised: a multi-classifier system which incorporates existing biological knowledge and returns interpretable models based on these high-level semantic terms.
References
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Journal ArticleDOI
Laminin--a glycoprotein from basement membranes.
Rupert Timpl,H Rohde,Pamela Gehron Robey,Stephen I. Rennard,Jean-Michel Foidart,George R. Martin +5 more
TL;DR: Investigation of a large noncollagenous glycoprotein isolated from a mouse tumor that produces basement membrane shows that it is produced by a variety of cultured cells, suggesting that this protein or an immunologically related protein is a constituent of the basement membranes of these tissues.
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Structure and function of leukocyte integrins.
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Journal ArticleDOI
Regulated expression and binding of three VLA (β1) integrin receptors on T cells
TL;DR: The regulated binding of resting CD4+ human T cells to ECM through three VLA integrins is described, including a novel pathway of VLA-6 binding to laminin (LN).
Journal ArticleDOI
Cytokine-induced respiratory burst of human neutrophils: dependence on extracellular matrix proteins and CD11/CD18 integrins.
Carl Nathan,Subita Srimal,Charles Farber,Elizabeth Sanchez,Lynda Kabbash,Adam S. Asch,James Gailit,Samuel D. Wright +7 more
TL;DR: For cytokines to induce a respiratory burst in PMN, the cells must be able to use CD11/CD18 integrins and must interact with matrix proteins in the solid phase, and receptor(s) exist on PMN for fibronectin and vitronECTin which are not blocked by the anti-CD18 antibody IB4 but which are nonetheless CD11 /CD18 dependent.
Book ChapterDOI
Fibronectin: purification, immunochemical properties, and biological activities.
TL;DR: Fibronectin is a cell-surface and blood glycoprotein that apparently mediates adhesion of cells to the extracellular matrix that may contribute to their capacity for invasive and metastatic growth.