Increased leaf size: different means to an end
Nathalie Gonzalez,Stefanie De Bodt,Ronan Sulpice,Yusuke Jikumaru,Eunyoung Chae,Stijn Dhondt,Twiggy Van Daele,Liesbeth De Milde,Detlef Weigel,Yuji Kamiya,Mark Stitt,Gerrit T.S. Beemster,Dirk Inzé +12 more
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TLDR
In this article, the authors performed a comparative analysis of transgenic lines that produce enlarged leaves under standardized environmental conditions and identified five genes belonging to different functional classes that all positively affect leaf size when overexpressed: AVP1, GRF5, JAW, BRI1 and GA20OX1.Abstract:
The final size of plant organs, such as leaves, is tightly controlled by environmental and genetic factors that must spatially and temporally coordinate cell expansion and cell cycle activity However, this regulation of organ growth is still poorly understood The aim of this study is to gain more insight into the genetic control of leaf size in Arabidopsis (Arabidopsis thaliana) by performing a comparative analysis of transgenic lines that produce enlarged leaves under standardized environmental conditions To this end, we selected five genes belonging to different functional classes that all positively affect leaf size when overexpressed: AVP1, GRF5, JAW, BRI1, and GA20OX1 We show that the increase in leaf area in these lines depended on leaf position and growth conditions and that all five lines affected leaf size differently; however, in all cases, an increase in cell number was, entirely or predominantly, responsible for the leaf size enlargement By analyzing hormone levels, transcriptome, and metabolome, we provide deeper insight into the molecular basis of the growth phenotype for the individual lines A comparative analysis between these data sets indicates that enhanced organ growth is governed by different, seemingly independent pathways The analysis of transgenic lines simultaneously overexpressing two growth-enhancing genes further supports the concept that multiple pathways independently converge on organ size control in Arabidopsisread more
Citations
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Leaf size control: complex coordination of cell division and expansion.
TL;DR: The current understanding of the cellular and molecular mechanisms that govern leaf organ size are reviewed and future prospects on research aiming at understanding organ size regulation are discussed.
Journal ArticleDOI
Exit from Proliferation during Leaf Development in Arabidopsis thaliana: A Not-So-Gradual Process
Megan Andriankaja,Stijn Dhondt,Stefanie De Bodt,Hannes Vanhaeren,Frederik Coppens,Liesbeth De Milde,Per Mühlenbock,Aleksandra Skirycz,Nathalie Gonzalez,Gerrit T.S. Beemster,Gerrit T.S. Beemster,Dirk Inzé +11 more
TL;DR: Genes differentially expressed during the transition from cell proliferation to expansion were enriched in genes involved in cell cycle, photosynthesis, and chloroplast retrograde signaling, and differentiation of the photosynthetic machinery is important for regulating the exit from proliferation.
Journal ArticleDOI
Decline of Leaf Hydraulic Conductance with Dehydration: Relationship to Leaf Size and Venation Architecture
TL;DR: Hydraulic vulnerability was lower with greater major vein density and smaller leaf size, pointing to a new functional role of venation architecture and small leaf size in drought tolerance, potentially contributing to well-known biogeographic trends in leaf size.
Journal ArticleDOI
Cell to whole-plant phenotyping: the best is yet to come.
TL;DR: Overall, attention is focused on spatial and temporal resolution because these are crucial aspects of imaging procedures in plant phenotyping systems.
Journal ArticleDOI
SPEECHLESS integrates brassinosteroid and stomata signalling pathways.
Gustavo Eduardo Gudesblat,Joanna Schneider-Pizoń,Camilla Betti,Juliane Mayerhofer,Isabelle Vanhoutte,Walter van Dongen,Sjef Boeren,Miroslava Zhiponova,Miroslava Zhiponova,Sacco C. de Vries,Claudia Jonak,Eugenia Russinova +11 more
TL;DR: It is demonstrated that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.
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