Influence of the abstinence period on human sperm quality: analysis of 2,458 semen samples.
Vanessa A. Comar,Claudia G. Petersen,Ana L. Mauri,Mariana Mattila,Laura D. Vagnini,Adriana Renzi,Bruna Petersen,A. Nicoletti,Felipe Dieamant,Joao Batista A. Oliveira,Ricardo L. R. Baruffi,Jose G. Franco +11 more
TLDR
This study reinforces the importance of the duration of ejaculatory abstinence on semen parameter variation and highlights the deleterious effect of increased abstinence on DNA damage, which is most likely associated with ROS (mitochondrial damage/number of leukocytes).Abstract:
Objective The aim of this study was to evaluate the influence of different periods of abstinence on conventional semen parameters as well as functional parameters in human semen, including mitochondrial function, chromatin packing and sperm DNA fragmentation. Methods We recruited a cohort of 2,458 men undergoing infertility investigation. Semen analyses were performed according to WHO guidelines/morphology-motile sperm organelle morphology examination/MSOME. For DNA integrity analysis, the percentages of DNA fragmentation (TUNEL), abnormal chromatin packaging/underprotamination (chromomycin A3/CMA3), abnormal mitochondrial membrane potential (MMP/MitoTracker Green), and apoptosis (annexin-V) were recorded. Associations between the sexual abstinence period and sperm parameters were assessed using Spearman correlation. For group comparisons, the subjects were categorized according to the sexual abstinence period (SAP) into three groups: SAP 5 days. Results The duration of abstinence had a statistically significant positive influence on sperm concentration and volume, the number of leukocytes and a statistically significant negative influence on sperm motility and vitality. The percentages of DNA fragmentation and MMP (mitochondrial damage) worsened with the increased duration of abstinence. The percentage of sperm protamination was statistically significantly increased with abstinence. Conclusion Increase in the sexual abstinence period influences sperm quality. This study reinforces the importance of the duration of ejaculatory abstinence on semen parameter variation. It highlights the deleterious effect of increased abstinence on DNA damage, which is most likely associated with ROS (mitochondrial damage/number of leukocytes). The increase in chromatin packaging can represent a protective feature for DNA.read more
Citations
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Current Insights and Latest Updates in Sperm Motility and Associated Applications in Assisted Reproduction
Reyon Dcunha,Reda S. Hussein,Reda S. Hussein,Hanumappa Ananda,Sandhya Kumari,Satish Kumar Adiga,Nagarajan Kannan,Yulian Zhao,Guruprasad Kalthur,Guruprasad Kalthur +9 more
TL;DR: Various pharmacological agents and biomolecules that can enhance sperm motility in vitro and in vivo conditions to improve assisted reproductive technology (ART) outcomes are discussed.
Journal ArticleDOI
Should we be measuring DNA damage in human spermatozoa? New light on an old question.
R. John Aitken,Hassan W. Bakos +1 more
TL;DR: It is argued that infertility specialists should be engaged in the diagnosis and remediation of sperm DNA damage as a matter of best practice, in order to minimize the risk of adverse health outcomes in children conceived using ART.
Journal ArticleDOI
Investigating the Glycating Effects of Glucose, Glyoxal and Methylglyoxal on Human Sperm
TL;DR: The observed detrimental effects of the increased levels of AGE on DNA integrity, without an effect on motility and hyaluronidase activity, suggest that sperm may retain some fertilizing capacity under these adverse conditions.
Journal ArticleDOI
Diabetes and Sperm DNA Damage: Efficacy of Antioxidants
Nagarajan Laleethambika,Venugopal Anila,Chandran Manojkumar,Ishvarya Muruganandam,Bupesh Giridharan,Thangarasu Ravimanickam,Vellingiri Balachandar +6 more
TL;DR: The potential impact of DM on sperm DNA integrity, epigenetic dysregulation and efficacy of antioxidant therapy is highlighted and Administration of antioxidants in male infertility has started to pull in significant intrigue.
Journal ArticleDOI
The longitudinal effect of ejaculation on seminal vesicle fluid volume and whole-prostate ADC as measured on prostate MRI
TL;DR: Three-day abstinence from ejaculation is required to ensure maximal seminal vesicle distension and significant reductions in SV volume and whole-gland ADC were observed post-ejaculation, supporting a 3-day period of abstinence before prostate MRI.
References
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Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic
Donald P. Evenson,Lorna K. Jost,D Marshall,M J Zinaman,E D Clegg,K Purvis,P. De Angelis,O P Claussen +7 more
TL;DR: Based on logistic regression, spermatozoa with denatured DNA (cells outside the main population, COMP alpha t) were the best predictor for whether a couple would not achieve pregnancy.
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Role of sperm chromatin abnormalities and DNA damage in male infertility
Ashok Agarwal,Tamer M. Said +1 more
TL;DR: Screening for sperm DNA damage may provide useful information in cases of male idiopathic infertility and in those men pursuing assisted reproduction, and treatment should include methods for prevention of spermDNA damage.
Journal ArticleDOI
Formation of reactive oxygen species in spermatozoa of infertile patients
Akira Iwasaki,Claude Gagnon +1 more
TL;DR: In this paper, the incidence of reactive oxygen species formation in whole semen and in washed spermatozoa was studied and the effect of the type of sperm washing was also investigated.
Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility
Ramadan A Saleh,Ramadan A Saleh,Ashok Agarwal,Essam A. Nada,Mohamed H El-Tonsy,Rakesh Sharma,Andrew Meyer,David R. Nelson,Anthony J. Thomas +8 more
TL;DR: In this article, the effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility were examined in a tertiary care academic institution.
Journal ArticleDOI
Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility
Ramadan A Saleh,Ramadan A Saleh,Ashok Agarwal,Essam A. Nada,Mohamed H El-Tonsy,Rakesh Sharma,Andrew Meyer,David R. Nelson,Anthony J. Thomas +8 more
TL;DR: In this paper, the effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility were examined in a tertiary care academic institution.