Journal ArticleDOI
Inheritance of a pre-inactivated paternal X chromosome in early mouse embryos
Khanh D. Huynh,Jeannie T. Lee +1 more
TLDR
It is argued that the XX embryo is in fact dosage compensated at conception along much of the X chromosome, and proposed that imprinted X inactivation results from inheritance of a pre-inactivated X chromosome from the paternal germ line.Abstract:
In mammals, dosage compensation ensures equal X-chromosome expression between males (XY) and females (XX) by transcriptionally silencing one X chromosome in XX embryos In the prevailing view, the XX zygote inherits two active X chromosomes, one each from the mother and father, and X inactivation does not occur until after implantation Here, we report evidence to the contrary in mice We find that one X chromosome is already silent at zygotic gene activation (2-cell stage) This X chromosome is paternal in origin and exhibits a gradient of silencing Genes close to the X-inactivation centre show the greatest degree of inactivation, whereas more distal genes show variable inactivation and can partially escape silencing After implantation, imprinted silencing in extraembryonic tissues becomes globalized and more complete on a gene-by-gene basis These results argue that the XX embryo is in fact dosage compensated at conception along much of the X chromosome We propose that imprinted X inactivation results from inheritance of a pre-inactivated X chromosome from the paternal germ lineread more
Citations
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Journal ArticleDOI
X-inactivation profile reveals extensive variability in X-linked gene expression in females
TL;DR: A comprehensive X-inactivation profile of the human X chromosome is presented, representing an estimated 95% of assayable genes in fibroblast-based test systems, and suggests a remarkable and previously unsuspected degree of expression heterogeneity among females.
Journal ArticleDOI
Single-cell RNA-seq reveals dynamic, random monoallelic gene expression in mammalian cells.
Qiaolin Deng,Daniel Ramsköld,Daniel Ramsköld,Björn Reinius,Björn Reinius,Rickard Sandberg,Rickard Sandberg +6 more
TL;DR: It is concluded that independent and stochastic allelic transcription generates abundant random monoallelic expression in the mammalian cell.
Journal ArticleDOI
Epigenetic events in mammalian germ-cell development: reprogramming and beyond
Hiroyuki Sasaki,Yasuhisa Matsui +1 more
TL;DR: It is shown that epigenetic modifiers have key roles in germ-cell development itself and contributes to the gene-expression programme that is required for germ- cell development, regulation of meiosis and genomic integrity.
Journal ArticleDOI
The key to development: interpreting the histone code?
TL;DR: Evidence is provided that the enzymes responsible for the modifications of histones function in a coordinated pattern to control gene expression in the short term and, through the transferral of these modifications by inheritance to their progeny, in the long term.
BookDOI
Long Noncoding RNAs
TL;DR: The chapter shows that the current understanding of what is a gene should be revised, in order to clearly define the complex relationship between product-coding regions, regulatory sequences, and the organism’s phenotype.
References
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Journal ArticleDOI
Gene Action in the X -chromosome of the Mouse ( Mus musculus L.)
TL;DR: Ohno and Hauschka1 showed that in female mice one chromosome of mammary carcinoma cells and of normal diploid cells of the ovary, mammary gland and liver was heteropyKnotic and suggested that the so-called sex chromatin was composed of one heteropyknotic X-chromosome.
Journal ArticleDOI
Demethylation of the zygotic paternal genome
TL;DR: It is shown that the paternal genome in the mouse is significantly and actively demethylated within 6–8 hours of fertilization, before the onset of DNA replication, whereas the maternal genome is dem methylated after several cleavage divisions.
Journal ArticleDOI
Promotion of Trophoblast Stem Cell Proliferation by FGF4
TL;DR: A culture of mouse blastocysts or early postimplantation trophoblasts in the presence of fibroblast growth factor 4 (FGF4) permitted the isolation of permanent trophoblast stem cell lines that exclusively contributed to the trophoplast lineage in vivo in chimeras.
Journal ArticleDOI
Preferential inactivation of the paternally derived X chromosome in the extraembryonic membranes of the mouse
Nobuo Takagi,Motomichi Sasaki +1 more
TL;DR: In an effort to determine the embryonic stage at which the X chromosome initiates differentiation in famale mouse embryos heterozygous for Cattanach's translocaton, it was found that the mosaic composition was consistently biased in extraembryonic membranes, whereas it was not necessarily so in the embryonic body.
Journal ArticleDOI
Xist-deficient mice are defective in dosage compensation but not spermatogenesis.
TL;DR: The results indicate that the Xist RNA is required for female dosage compensation but plays no role in spermatogenesis.
Related Papers (5)
Epigenetic dynamics of imprinted X inactivation during early mouse development.
Preferential inactivation of the paternally derived X chromosome in the extraembryonic membranes of the mouse
Nobuo Takagi,Motomichi Sasaki +1 more