Open AccessJournal Article
Inhibition of Leishmania major PTR1 Gene Expression by Antisense in Escherichia coli.
Farnaz Kheirandish,Mojgan Bandehpour,Ali Haghighi,Fereidoun Mahboudi,M Mohebali,Bahram Kazemi +5 more
TLDR
In inhibition of Iranian L. coli strain M15, major PTR1 expression with mRNA antisense in prokaryotic system as an approach to appear of the drugs therapeutic effects more.Abstract:
Background: Protozoa related to Trypanosome family including Leishmania , synthesize enzymes to escape from drug therapy. One of them is PTR1 that its enzymatic activity is similar to dihydrofolate reductase (DHFR). Dihydrofolate reductase - thymidylate synthase has a major role in DNA synthesis, if it is inhibited, the result would be the death of parasite. Since PTR1 activity is similar to DHFR, causes the decrease of inhibition effect of drug. The aim of this study was inhibition of Iranian L. major PTR1 expression with mRNA antisense in prokaryotic system as an approach to appear of the drugs therapeutic effects more. Methods: PTR1 gene was ligated to pACYCDuet-1 and pcDNA3 plasmids as sense and antisense plasmids, respectively. Simultaneously transfer of sense and antisense plasmids was done in E. coli strain M15. SDS-PAGE and western blot analysis were carried out to analyze the expression. Results: Sense and antisense plasmids were prepared and confirmed by restriction analysis and PCR then simultaneously transfer of them was done. SDS-PAGE and western blot analysis showed PTR1 gene was inhibited by mRNA antisense in bacterial cells. Conclusion: Expression of PTR1 gene in sense plasmid was inhibited successfully by antisense plasmid.read more
Citations
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Antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier extract.
Farnaz Kheirandish,Bahram Delfan,Hossein Mahmoudvand,Nasim Moradi,Behrouz Ezatpour,Farzad Ebrahimzadeh,Marzieh Rashidipour +6 more
TL;DR: In this article, the authors evaluated the antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier (oak) extract and found that oak significantly inhibited the growth rate of promastigote and amastigotes.
Journal ArticleDOI
Leishmania treatment and prevention: Natural and synthesized drugs.
Adnan A. Bekhit,Adnan A. Bekhit,Eman J. El-Agroudy,Aliaa Helmy,Tamer M. Ibrahim,Amin Shavandi,Alaa El-Din A. Bekhit +6 more
TL;DR: This review focuses on recent findings in drugs used for the treatment of leishmaniasis including; chemical and natural antileishmanial moieties, different potential targets, as well as various trials of vaccination development.
Journal ArticleDOI
In-Silico Analyses of Sesquiterpene-Related Compounds on Selected Leishmania Enzyme-Based Targets
TL;DR: Through molecular docking it was found that two sesquiterpene coumarins are promising leads for the P TR1 and TryS inhibition purposes, and some xanthanolides also exhibited better affinity towards PTR1 and CS binding.
Journal ArticleDOI
Hybridization of different antisense oligonucleotides on the surface of gold nanoparticles to silence zinc metalloproteinase gene after uptake by Leishmania major
TL;DR: It could be concluded that both sequence and size of antisense oligonucleotide were important for transfection of L. major and hybridized gold nanoparticles not only could silence GP63 gene, but also could kill L.major.
Journal ArticleDOI
How often do children receive their vaccinations late, and why?
Maysaa A. Banjari,Ahmed Abdullah Alamri,Ahmad Y. Algarni,Meral Hassan Abualjadayel,Yahya S. Alshardi,Turki S Alahmadi +5 more
TL;DR: Adherence to vaccination is fairly common in this part of the country, however, vaccination delays are still present and should be addressed to improve health care.
References
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Journal Article
Molecular Cloning and Expression of Iranian Leishmania major Pteridine Reductase 1
F Kheirandish,M Bandehpour,Ali Haghighi,Fereidoun Mahboudi,Mehdi Mohebali,Nariman Mosaffa,Bahram Kazemi +6 more
TL;DR: This enzyme will be used for further investigation about Leishmania antifolate therapy that is effective against PTR1 and is sequenced and deposited in GenBank.
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