Open AccessJournal Article
Inhibition of Leishmania major PTR1 Gene Expression by Antisense in Escherichia coli.
Farnaz Kheirandish,Mojgan Bandehpour,Ali Haghighi,Fereidoun Mahboudi,M Mohebali,Bahram Kazemi +5 more
TLDR
In inhibition of Iranian L. coli strain M15, major PTR1 expression with mRNA antisense in prokaryotic system as an approach to appear of the drugs therapeutic effects more.Abstract:
Background: Protozoa related to Trypanosome family including Leishmania , synthesize enzymes to escape from drug therapy. One of them is PTR1 that its enzymatic activity is similar to dihydrofolate reductase (DHFR). Dihydrofolate reductase - thymidylate synthase has a major role in DNA synthesis, if it is inhibited, the result would be the death of parasite. Since PTR1 activity is similar to DHFR, causes the decrease of inhibition effect of drug. The aim of this study was inhibition of Iranian L. major PTR1 expression with mRNA antisense in prokaryotic system as an approach to appear of the drugs therapeutic effects more. Methods: PTR1 gene was ligated to pACYCDuet-1 and pcDNA3 plasmids as sense and antisense plasmids, respectively. Simultaneously transfer of sense and antisense plasmids was done in E. coli strain M15. SDS-PAGE and western blot analysis were carried out to analyze the expression. Results: Sense and antisense plasmids were prepared and confirmed by restriction analysis and PCR then simultaneously transfer of them was done. SDS-PAGE and western blot analysis showed PTR1 gene was inhibited by mRNA antisense in bacterial cells. Conclusion: Expression of PTR1 gene in sense plasmid was inhibited successfully by antisense plasmid.read more
Citations
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Antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier extract.
Farnaz Kheirandish,Bahram Delfan,Hossein Mahmoudvand,Nasim Moradi,Behrouz Ezatpour,Farzad Ebrahimzadeh,Marzieh Rashidipour +6 more
TL;DR: In this article, the authors evaluated the antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier (oak) extract and found that oak significantly inhibited the growth rate of promastigote and amastigotes.
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Leishmania treatment and prevention: Natural and synthesized drugs.
Adnan A. Bekhit,Adnan A. Bekhit,Eman J. El-Agroudy,Aliaa Helmy,Tamer M. Ibrahim,Amin Shavandi,Alaa El-Din A. Bekhit +6 more
TL;DR: This review focuses on recent findings in drugs used for the treatment of leishmaniasis including; chemical and natural antileishmanial moieties, different potential targets, as well as various trials of vaccination development.
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In-Silico Analyses of Sesquiterpene-Related Compounds on Selected Leishmania Enzyme-Based Targets
TL;DR: Through molecular docking it was found that two sesquiterpene coumarins are promising leads for the P TR1 and TryS inhibition purposes, and some xanthanolides also exhibited better affinity towards PTR1 and CS binding.
Journal ArticleDOI
Hybridization of different antisense oligonucleotides on the surface of gold nanoparticles to silence zinc metalloproteinase gene after uptake by Leishmania major
TL;DR: It could be concluded that both sequence and size of antisense oligonucleotide were important for transfection of L. major and hybridized gold nanoparticles not only could silence GP63 gene, but also could kill L.major.
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How often do children receive their vaccinations late, and why?
Maysaa A. Banjari,Ahmed Abdullah Alamri,Ahmad Y. Algarni,Meral Hassan Abualjadayel,Yahya S. Alshardi,Turki S Alahmadi +5 more
TL;DR: Adherence to vaccination is fairly common in this part of the country, however, vaccination delays are still present and should be addressed to improve health care.
References
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Book
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New approaches to Leishmania chemotherapy: pteridine reductase 1 (PTR1) as a target and modulator of antifolate sensitivity
TL;DR: Findings suggest that successful antifolate chemotherapy in Leishmania will have to target simultaneously both DHFR and PTR1, which has the potential to act as a by-pass and/or modulator of DHFR inhibition under physiological conditions.
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TL;DR: The data suggest that the mechanism of MTX cross-resistance associated with H region amplification is novel and distinct from that mediated by overexpression of MDR genes in multidrug-resistant mammalian cells.
Journal ArticleDOI
Pteridine salvage throughout the Leishmania infectious cycle: implications for antifolate chemotherapy.
TL;DR: Pteridine salvage by amastigotes may account for the clinical inefficacy of antifolates against leishmaniasis, and ultimately provide insights into how this may be overcome in the future.
Journal ArticleDOI
A randomized, placebo-controlled trial of a two-week regimen of aminosidine (paromomycin) ointment for treatment of cutaneous leishmaniasis in Iran.
TL;DR: It is concluded that this twice a day two-week regimen of aminosidine was inadequate to accelerate the recovery of most cases of cutaneous leishmaniasis, but the ointment did show some clear evidence of parasitologic efficacy and should now be studied in longer or more frequent regimens in an effort to prevent Parasitologic relapse and thus promote clinical improvement.
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