Interactions between RNA polymerase II, factors, and template leading to accurate transcription.
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TLDR
A reconstituted transcription system, consisting of purified RNA polymerase II and three essential HeLa cell chromatographic fractions, is used to study events leading to transcription from the adenovirus major late promoter.About:
This article is published in Journal of Biological Chemistry.The article was published on 1984-02-25 and is currently open access. It has received 204 citations till now. The article focuses on the topics: Transcription factor II D & RNA polymerase II.read more
Citations
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Journal ArticleDOI
Steroid Receptor Regulated Transcription of Specific Genes and Gene Networks
TL;DR: Information is provided on how to identify the Steroid Receptors, Receptor Binding Sites, and other mechanisms that aid in the identification of the receptors and their locations in the genome.
Journal ArticleDOI
Tissue-specific in vitro transcription from the mouse albumin promoter
K. Gorski,Carneiro M,U. Schibler +2 more
TL;DR: Transcriptionally active nuclear extracts have been prepared from rat liver, brain, and spleen and the adenovirus-2 major late promoter directs efficient transcription by RNA polymerase II in all of these extracts, whereas the promoter of the mouse albumin gene is significantly used only in the liver extract.
Journal ArticleDOI
Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins
TL;DR: The enzyme that carries out RNA synthesis provides the cell with the means to adjust the patterns of transcription in response to environmental and developmental signals, and the presence of promoter-specific transcription factors enables other components of the transcriptional machinery, including the RNA polymerase, to initiate transcription with selectivity and accuracy.
Journal ArticleDOI
Five intermediate complexes in transcription initiation by RNA polymerase II
TL;DR: A native gel electrophoresis DNA binding assay was used to resolve complexes formed on the adenovirus Major Late Promoter by general transcription factors and RNA polymerase II, generating new complexes that contained accurately initiated transcripts associated with the transcription machinery and the template DNA.
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The general transcription machinery and general cofactors.
Mary C. Thomas,Cheng Ming Chiang +1 more
TL;DR: These cofactors are capable of repressing basal transcription when activators are absent and stimulating transcription in the presence of activators, with emphasis on the events occurring after the chromatin has been remodeled but prior to the formation of the first phosphodiester bond.
References
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DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract
TL;DR: A cell-free system for studying the synthesis of mRNA in mammalian cells, which consists of a dialyzed and concentrated whole-cell extract derived from HeLa cells, small molecules and cofactors needed for transcription, and exogenously added DNA.
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Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II
William S. Dynan,Robert Tjian +1 more
TL;DR: Addition of the Sp1 factor stimulated SV40 early promoter transcription 40-fold, and inhibited adenovirus major late promoter transcription by 40%.
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Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes
TL;DR: A factor necessary for the accurate transcription of cloned Xenopus 5S genes in vitro has been isolated from soluble extracts of X. laevis ovaries and the site of action has been investigated using the "footprinting" method of Galas and Schmitz (1978).
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Selective and accurate initiation of transcription at the ad2 major late promotor in a soluble system dependent on purified rna polymerase ii and dna
TL;DR: Specific transcription initiation at the late promoter is observed with extracts derived from either virus-infected or uninfected KB cells and with class II RNA polymerases isolated from either human calf, murine or amphibian cells.
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Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
TL;DR: A soluble extract prepared from human cells (KB S-100) has been recently shown to direct accurate transcription initiation by purified RNA polymerase II at the major late promoter of adenovirus 2, and four components that are required for the active and selective initiation of transcription by RNA polymerases II at this promoter are identified.