Isolation and characterization of a mouse y chromosomal repetitive sequence
Yutaka Nishioka,Estelle Lamothe +1 more
TLDR
Based on the hybridization patterns against mouse Y chromosomal DNA, AC11 classified 16 inbred laboratory strains into two categories; those with the Mus musculus musculus type Y chromosome and those without, indicating that the amplification of AC11-related sequences in the mouse Y chromosome was a recent evolutionary event.Abstract:
The Y chromosome plays a dominant role in mammalian sex determination, and characterization of this chromosome is essential to understand the mechanism responsible for testicular differentiation. Male mouse genomic DNA fragments, cloned into pBR322, were screened for the presence of Bkm (a female snake satellite DNA)-related sequences, and we obtained a clone (AC11) having a DNA fragment from the mouse Y chromosome. In addition to a Bkm-related sequence, this fragment contained a Y chromosomal repetitive sequence. DNA isolated from the XX sex-reversed male genome produced a hybridization pattern indistinguishable to that obtained with normal female DNA, suggesting that the AC11 sequence is not contained within the Y chromosomal DNA present in the sex-reversed male genome. Based on the hybridization patterns against mouse Y chromosomal DNA, AC11 classified 16 inbred laboratory strains into two categories; those with the Mus musculus musculus type Y chromosome and those with the M.m. domesticus type Y chromosome. Three European subspecies of Mus musculus (M.m. brevirostris, M.m. poschiavinus and M.m. praetextus) possessed the M.m. domesticus type Y chromosome, whereas the Japanese mouse, M.m. molossinus, had the M.m. musculus type Y chromosome. The survey was also extended to six other species that belong to the genus Mus, of which M. spretus and M. hortulamus showed significant amounts of AC11-related sequences in their Y chromosomes. The male-specific accumulation of AC11-related sequences was not found in M. caroli, M. cookii, M. pahari or M. platythrix. This marked difference among Mus species indicates that the amplification of AC11-related sequences in the mouse Y chromosome was a recent evolutionary event.read more
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Journal ArticleDOI
Sequencing the Mouse Y Chromosome Reveals Convergent Gene Acquisition and Amplification on Both Sex Chromosomes
Y. Q. Shirleen Soh,Jessica Alföldi,Tatyana Pyntikova,Laura G. Brown,Laura G. Brown,Tina Graves,Patrick Minx,Robert S. Fulton,Colin Kremitzki,Natalia Koutseva,Jacob L. Mueller,Steve Rozen,Jennifer F. Hughes,Elaine Owens,James E. Womack,William J. Murphy,Qing Cao,Pieter J. de Jong,Wesley C. Warren,Richard K. Wilson,Helen Skaletsky,Helen Skaletsky,David C. Page,David C. Page +23 more
TL;DR: The complete mouse MSY sequence brings to light dramatic forces in sex chromosome evolution: lineage-specific convergent acquisition and amplification of X-Y gene families, possibly fueled by antagonism between acquired X-y homologs.
Journal ArticleDOI
Hypertension in the Spontaneously Hypertensive Rat is Linked to the Y-Chromosome
Daniel Ely,Monte E. Turner +1 more
TL;DR: The data strongly suggest that hypertension in the SHR has two primary components of equal magnitude, one consisting of a small number of autosomal loci with a second Y-linked component.
Journal ArticleDOI
A New Deletion of the Mouse Y Chromosome Long Arm Associated With the Loss of Ssty Expression, Abnormal Sperm Development and Sterility
Aminata Touré,Maria Szot,Maria Szot,Shantha K. Mahadevaiah,Áine Rattigan,Obah A. Ojarikre,Paul S. Burgoyne +6 more
TL;DR: It is established that genetic information essential for normal sperm differentiation and function is present on mouse Yq, and a more extensive Yq deletion that abolishes Ssty expression and results in severe sperm defects and sterility is reported.
Journal ArticleDOI
Identification of novel Y chromosome encoded transcripts by testis transcriptome analysis of mice with deletions of the Y chromosome long arm
Aminata Touré,Emily J Clemente,Peter J.I. Ellis,Shantha K. Mahadevaiah,Obah A. Ojarikre,Penelope Alexandra Falshaw Ball,Louise N. Reynard,Kate L Loveland,Paul S. Burgoyne,Nabeel A. Affara +9 more
TL;DR: Further MSYq-encoded transcripts expressed in spermatids and deriving from multicopy Y genes, deficiency of which may underlie the defects in sperm development associated with MSYQ deletions are identified.
Journal ArticleDOI
Duplication, Deletion, and Polymorphism in the Sex-Determining Region of the Mouse Y Chromosome
Graeme Mardon,Rebecca Mosher,Christine M. Disteche,Yutaka Nishioka,Anne McLaren,David C. Page +5 more
TL;DR: The Y chromosomes of the subspecies Mus musculus musculus and M. m.
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