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Journal ArticleDOI

Isolation and characterization of a new thermophilic Methanosarcina strain (strain MP)

Bernard Ollivier, +2 more
- 01 Sep 1984 - 
- Vol. 135, Iss: 2, pp 187-198
TLDR
A thermophilic Methanosarcina strain was isolated from a digester fed with water hyacinths and inoculated with ground termites from the Congo and grew on acetate, methanol and methylamines in the absence of growth factors, but could not use H2-CO2 or formate.
Abstract
A thermophilic Methanosarcina strain was isolated from a digester fed with water hyacinths and inoculated with ground termites from the Congo. Optimal growth temperature was 55 degrees C. Methane production was at its optimum between pH 6.5 and 7.0. The bacterium grew on acetate, methanol and methylamines in the absence of growth factors, but could not use H2-CO2 or formate. H2-CO2 inhibited acetate utilisation. Yeast extract and vitamins stimulated growth.

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Citations
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Book ChapterDOI

Diversity and Taxonomy of Methanogens

TL;DR: This work has shown that methane production is a ubiquitous, defining characteristic of methanogens, a group of microbes that is phylogenetically distinct from eukaryotes and true bacteria.
Journal ArticleDOI

Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.

TL;DR: Strain TMOT was able to degrade methanol to CO2 and H2 in syntrophic culture with Methanothermobacter thermautotrophicus AH or Thermodesulfovibrio yellowstonii and is related to Thermotoga subterranea andThermotoga elfii.
Journal ArticleDOI

Limitations of thermophilic anaerobic wastewater treatment and the consequences for process design.

TL;DR: In this article, the process stability of thermophilic anaerobic wastewater treatment systems is investigated and a relatively low sensitivity to temperature changes if high-rate reactors with immobilized biomass are used.
Journal ArticleDOI

Temperature susceptibility of thermophilic methanogenic sludge: Implications for reactor start-up and operation

TL;DR: In this paper, the effect of temperature on the conversion rates of VFA by thermophilic methanogenic sludge grown under different conditions was studied, and the optimum temperatures for acetate degradation of sludges cultivated in serum bottles at 46, 55 and 64°C for 6-8 weeks were found to be strongly dependent on the cultivation temperature.
References
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Journal ArticleDOI

Isolation and characterization ofMethanococcus mazei

TL;DR: The present isolate of Methanococcus mazei strain S-6 exhibited a life cycle, and the forms produced during its development fit the morphological description of M. mazei exactly.
Book ChapterDOI

The Methanogenic Bacteria

TL;DR: The methanogenic bacteria are unique among pro-karyotesBecause they produce a hydrocarbon, methane, as a major product of anaerobic metabolism, their taxonomy has been obfuscated by the difficulty of obtaining members in pure culture.
Journal ArticleDOI

Bacterial methanogenesis: Acetate as a methane precursor in pure culture

TL;DR: Methyl labeled acetate was shown to be incorporated into whole cells and converted to 14CO2 and 14CH4 in the presence of H2 and 25 mM CO2/HCO3− and the importance of acetate utilization in microbial methanogenesis was discussed.
Journal ArticleDOI

Isolation and Characterization of an H2-Oxidizing Thermophilic Methanogen

TL;DR: A thermophilic methanogen was isolated from enrichment cultures originally inoculated with sludge from an anaerobic kelp digester and exhibited a temperature optimum of 55 to 60 degrees C and a maximum near 70 degrees C, and was sensitive to tetracycline and chloramphenicol but not penicillin G or cycloserine.
Journal ArticleDOI

Isolation and Characterization of a Thermophilic Marine Methanogenic Bacterium, Methanogenium thermophilicum sp. nov.†

TL;DR: A new species of thermophilic marine methanogenic bacteria is described, which occurred as irregular cocci, singly or in pairs, and did not possess flagella.
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