Open Access
Isolation and Characterization of Bacteria That Degrade Phosphonates in Marine Dissolved Organic Matter
Oscar A. Sosa,Daniel J. Repeta,Sara Ferrón,Jessica A. Bryant,Daniel R. Mende,David M. Karl,Edward F. DeLong +6 more
TLDR
Bacteria isolated from the North Pacific Subtropical Gyre (NPSG) near Hawaii that are able to degrade phosphonates associated with high molecular weight dissolved organic matter (HMWDOM) are described and it is postulate that the complete remineralization of HMWDOM polysaccharides requires more complex microbial inter-species interactions.Abstract:
Semi-labile dissolved organic matter (DOM) accumulates in surface waters of the oligotrophic ocean gyres and turns over on seasonal to annual timescales. This reservoir of DOM represents an important source of carbon, energy, and nutrients to marine microbial communities but the identity of the microorganisms and the biochemical pathways underlying the cycling of DOM remain largely uncharacterized. In this study we describe bacteria isolated from the North Pacific Subtropical Gyre (NPSG) near Hawaii that are able to degrade phosphonates associated with high molecular weight dissolved organic matter (HMWDOM), which represents a large fraction of semi-labile DOM. We amended dilution-to-extinction cultures with HMWDOM collected from NPSG surface waters and with purified HMWDOM enriched with polysaccharides bearing alkylphosphonate esters. The HMWDOM-amended cultures were enriched in Roseobacter isolates closely related to Sulfitobacter and close relatives of hydrocarbon-degrading bacteria of the Oceanospirillaceae family, many of which encoded phosphonate degradation pathways. Sulfitobacter cultures encoding C-P lyase were able to catabolize methylphosphonate and 2-hydroxyethylphosphonate, as well as the esters of these phosphonates found in native HMWDOM polysaccharides to acquire phosphorus while producing methane and ethylene, respectively. Conversely, growth of these isolates on HMWDOM polysaccharides as source of carbon did not support robust increases in cell yields, suggesting that the constituent carbohydrates in HMWDOM were not readily available to these individual isolates. We postulate that the complete remineralization of HMWDOM polysaccharides requires more complex microbial inter-species interactions. The degradation of phosphonate esters and other common substitutions in marine polysaccharides may be key steps in the turnover of marine DOM.read more
Citations
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SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)
TL;DR: SPAdes as mentioned in this paper is a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler and on popular assemblers Velvet and SoapDeNovo (for multicell data).
Dissertation
Taxonomic and functional exploration of the biosphere of serpentinizing hydrothermal systems by metagenomics
TL;DR: In this article, les communautes microbiennes ont ete etudiees en se focalisant sur Prony, un ecosysteme serpentinise cotier de Nouvelle-Caledonie, en comparant differents ecosystemes serpentinises, pour faire emerger des similarites taxonomiques and fonctionnelles.
Metatranscriptomic and functional metagenomic analysis of methylphosphonate utilization by marine bacteria
TL;DR: In this paper, the authors identify specific bacterioplankton taxa that can utilize MPn aerobically under conditions of P limitation using the C-P lyase pathway, and thereby elicit a significant increase in the dissolved methane concentration.
MonographDOI
Microbial cycling of marine high molecular weight dissolved organic matter
TL;DR: In this article, the authors identify marine microorganisms that can serve as model systems to study the metabolic pathways and biochemical reactions that control an important ecosystem function, DOMturnover, by enriching seawater in dilution-to-extinction culturing with a natural source of DOM.
References
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