Journal ArticleDOI
Isolation, characterization, and chondrogenic potential of human bone marrow-derived multipotential stromal cells.
TLDR
An efficient and reproducible procedure for the isolation of stromal cells from bone marrow aspirates of normal human donors based on the expression of endoglin, a type III receptor of the transforming growth factor‐beta (TGF‐β) receptor family is developed.Abstract:
Multipotential bone marrow stromal cells have the ability to differentiate along multiple connective tissue lineages including cartilage. In this study, we developed an efficient and reproducible procedure for the isolation of stromal cells from bone marrow aspirates of normal human donors based on the expression of endoglin, a type III receptor of the transforming growth factor-beta (TGF-β) receptor family. We demonstrate that these cells have the ability of multiple lineage differentiation. Stromal cells represented 2–3% of the total mononuclear cells of the marrow. The cells displayed a fibroblastic colony formation in monolayer culture and maintained similar morphology with passage. Expression of cell surface molecules by flow cytometry displayed a stable phenotype with culture expansion. When cocultured with hematopoietic CD34+ progenitor cells, stromal cells were able to maintain their ability to support hematopoiesis in vitro. Culture expanded stromal cells were placed in a 3-dimensional matrix of alginate beads and cultured in serum-free media in the presence of TGFβ-3 for chondrogenic lineage progression. Increased expression of type II collagen messenger RNA was observed in the TGFβ3 treated cultures. Immunohistochemistry performed on sections of alginate beads detected the presence of type II collagen protein. This isolation procedure for stromal cells and the establishment of the alginate culture system for chondrogenic progression will contribute to the understanding of chondrogenesis and cartilage repair. J. Cell. Physiol. 185:98–106, 2000. © 2000 Wiley-Liss, Inc.read more
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Journal ArticleDOI
Surface protein characterization of human adipose tissue-derived stromal cells
Stan Gronthos,Dawn M. Franklin,Holly A. Leddy,Pamela Gehron Robey,Robert W. Storms,Jeffrey M. Gimble +5 more
TL;DR: Flow cytometry and immunohistochemistry show that human adipose tissue‐derived stromal cells have a protein expression phenotype that is similar to that of human bone marrow stroma cells.
Journal ArticleDOI
Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds.
TL;DR: The findings of this study suggest that various biomaterials support the chondrogenic differentiation of hADAS cells, and that manipulating the composition of these tissue engineered constructs may have significant effects on their mechanical properties.
Journal ArticleDOI
Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency
Rebecca J. McMurray,Nikolaj Gadegaard,P. Monica Tsimbouri,Karl Burgess,Laura E. McNamara,Rahul S. Tare,Kate Murawski,Emmajayne Kingham,Richard O.C. Oreffo,Richard O.C. Oreffo,Matthew J. Dalby +10 more
TL;DR: The study identifies a nanostructured surface that retains stem-cell phenotype and maintains stem- cell growth over eight weeks, and implicates a role for small RNAs in repressing key cell signalling and metabolomic pathways, demonstrating the potential of surfaces as non-invasive tools with which to address the stem cell niche.
Journal ArticleDOI
Bone Tissue Engineering using human mesenchymal stem cells: effects of scaffold material and medium flow
Lorenz Meinel,Vassilis Karageorgiou,Robert Fajardo,Brian D. Snyder,Vivek R. Shinde-Patil,Ludwig Zichner,David L. Kaplan,Robert Langer,Gordana Vunjak-Novakovic +8 more
TL;DR: It is suggested that osteogenesis in cultured MSC can be modulated by scaffold properties and flow environment.
Journal ArticleDOI
Sonication-induced gelation of silk fibroin for cell encapsulation.
TL;DR: Sonication provides a useful new tool with which to initiate rapid sol-gel transitions, such as for cell encapsulation, with high ionic strength and temperature and low pH responsible for increasing gelation kinetics.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Integrins: versatility, modulation, and signaling in cell adhesion.
Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal ArticleDOI
In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells
TL;DR: A culture system that facilitates the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal progenitor cells has been developed in this article, where cells obtained in bone marrow aspirates were first isolated by monolayer culture and then transferred into tubes and allowed to form three-dimensional aggregates in a chemically defined medium.
Journal ArticleDOI
Conditions controlling the proliferation of haemopoietic stem cells in vitro.
TL;DR: A liquid culture system is described whereby proliferation of haemopoietic stem cells, production of granulocyte precursor cells (CFU‐C), and extensive granulopoiesis can be maintained in vitro for several months.