Isolation of vascular smooth muscle cells from a single murine aorta.
Jenna Lynn Ray,Rebecca L. Leach,Jean Marc Herbert,Mark L. Benson +3 more
- Vol. 23, Iss: 4, pp 185-188
TLDR
A technique for isolating smooth muscle cells from a single mouse aorta is described, particularly useful when material is limiting, as is frequently the case when genetically modified animals are being characterized.Abstract:
The vascular smooth muscle cell plays a significant role in many important cardiovascular disorders, and smooth muscle biology is therefore important to cardiovascular research. The mouse is critical to basic cardiovascular research, largely because techniques for genetic manipulation are more fully developed in the mouse than in any other mammalian species. We describe here a technique for isolating smooth muscle cells from a single mouse aorta. This technique is particularly useful when material is limiting, as is frequently the case when genetically modified animals are being characterized.read more
Citations
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Preparation and characterization of highly porous, biodegradable polyurethane scaffolds for soft tissue applications
TL;DR: Smooth muscle cells were filtration seeded in the scaffolds and it was shown that both scaffolds supported cell adhesion and growth, with smooth muscle cells growing more extensively in the PEUU scaffold.
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The knockout of miR-143 and -145 alters smooth muscle cell maintenance and vascular homeostasis in mice: correlates with human disease
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Preparation and rheological characterization of a gel form of the porcine urinary bladder matrix.
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Microintegrating smooth muscle cells into a biodegradable, elastomeric fiber matrix.
TL;DR: Electrospraying vascular smooth muscle cells (SMCs) concurrently with electrospinning a biodegradable, elastomeric poly(ester urethane)urea (PEUU) matrix embodies a novel tissue engineering approach that could be applied to fabricate high cell density elastic tissue mimetics, blood vessels or other cardiovascular tissues.
References
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The smooth muscle cell. II. Growth of smooth muscle in culture and formation of elastic fibers.
TL;DR: Investigations of the morphology of smooth muscle derived from the inner media and intima of immature guinea pig aorta and radioautographic observations of the ability of aortic smooth muscle to synthesize and secrete extracellular proteins demonstrate that this cell is a connective tissue synthetic cell.
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Urokinase and tissue-type plasminogen activator are required for the mitogenic and chemotactic effects of bovine fibroblast growth factor and platelet-derived growth factor-BB for vascular smooth muscle cells.
TL;DR: Results indicate that, at least in vitro, t-PA is an important element of the activity of PDGF-BB with regard to the proliferation and migration of SMC whereas u- PA is a key factor in the effect of bFGF on SMC.
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Inhibition of vascular smooth muscle cell proliferation in culture by pentosan polysulphate and related compounds
TL;DR: Pentosan polysulphate was tested in vitro as an inhibitor of rabbit aortic smooth muscle cell proliferation and its effects were compared with those of dextran sulphate, laminarin sulphate and heparin fractions, suggesting that they exert their effect by binding to the cell surface, and not by interacting with the growth factors in serum.
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Isolation and culture of smooth muscle cells from human umbilical cord arteries.
TL;DR: A short method is described for obtaining a large number of pure vascular smooth muscle cells in culture from human umbilical cord arteries digested twice by an enzyme mixture of collagenase, trypsin, elastase, and DNAase with addition of α-tosyl-lysyl chloromethane.