Lectin Complement Pathway Proteins in Healthy Individuals.
Anne Troldborg,Annette G. Hansen,Søren Hansen,Jens C. Jensenius,Kristian Stengaard-Pedersen,Steffen Thiel +5 more
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TLDR
There were gender differences for most proteins, whereas age did not seem to influence concentration, and there were significant differences in concentrations between serum and plasma for all proteins except for MBL and MASP‐3.Abstract:
Since the discovery of the lectin pathway of complement activation, numerous clinical cohorts have been examined for one or more proteins, with the intention of uncovering the functions of the proteins or with the aim of discovering new biomarkers or diagnostic tools. To unveil the abnormal, it is pivotal to know the normal. Our aim was to describe the concentrations of the 11 known proteins of the lectin pathway in serum and plasma and to uncover possible gender differences, age and diurnal variations, which must be taken into account for investigation in different cohorts. We examined the concentrations of all lectin pathway proteins mannan-binding lectin (MBL), H-ficolin, L-ficolin, M-ficolin, collectin-K1, collectin-L1, MBL-associated serine protease 2 (MASP-2), MASP-3, MBL-associated protein of 44 kDa (MAp44) and MAp19 in 300 Danish blood donors in serum and ethylenediamine tetraacetic acid (EDTA) plasma in established assays, and we further developed a new assay to measure MASP-1 in the same samples. We found significant differences in concentrations between serum and plasma for all proteins except for MBL and MASP-3. H-ficolin, M-ficolin and MAp19 displayed convincing diurnal variation. H-ficolin, in particular, halved from morning to the middle of the night. There were gender differences for most proteins, whereas age did not seem to influence concentration. The present study underlines the necessity of considering which material to use, correct matching and a trial design that takes the nature of the protein into account in order for the outcome of cohort studies to have significant relevance.read more
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Age and Sex-Associated Changes of Complement Activity and Complement Levels in a Healthy Caucasian Population
Mariana Gaya de Costa,Felix Poppelaars,Felix Poppelaars,Cees van Kooten,Tom Eirik Mollnes,Tom Eirik Mollnes,Tom Eirik Mollnes,Francesco Tedesco,Reinhard Würzner,Leendert A. Trouw,Lennart Truedsson,Mohamed R. Daha,Mohamed R. Daha,Anja Roos,Marc A. Seelen +14 more
TL;DR: Significant sex and age-related differences in complement levels and functionality in the healthy population are demonstrated and age and sex analysis should be taken into consideration when discussing complement-related pathologies and subsequent complement-targeted therapies.
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The Human Platelet as an Innate Immune Cell: Interactions Between Activated Platelets and the Complement System.
TL;DR: The role of limited complement activation in enhancing platelet activation is discussed and how complement-targeting drugs may provide opportunities for further dissecting the complex interactions between complement and platelets is considered.
Journal ArticleDOI
Progressive IgA Nephropathy Is Associated With Low Circulating Mannan-Binding Lectin–Associated Serine Protease-3 (MASP-3) and Increased Glomerular Factor H–Related Protein-5 (FHR5) Deposition
Nicholas R. Medjeral-Thomas,Anne Troldborg,Anne Troldborg,Nicholas Constantinou,Hannah J. Lomax-Browne,Annette G. Hansen,Michelle Willicombe,Charles D. Pusey,H. Terence Cook,Steffen Thiel,Matthew C. Pickering +10 more
TL;DR: The data indicate that circulating MASP-3 levels could be used as a biomarker of disease severity in IgAN and that glomerular staining for FHR5 could both indicate alternative complement pathway activation and be a tissue marker of Disease severity.
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Soluble defense collagens: Sweeping up immune threats.
TL;DR: This review will focus on the molecular mechanisms by which these proteins efficiently defend against immune threats and contribute to tissue repair.
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Analysis of Factor D Isoforms in Malpuech-Michels-Mingarelli-Carnevale Patients Highlights the Role of MASP-3 as a Maturase in the Alternative Pathway of Complement.
Rasmus Pihl,Lisbeth Jensen,Annette G. Hansen,Ida B. Thøgersen,Stephanie Andres,Frederik Dagnæs-Hansen,Konrad Oexle,Jan J. Enghild,Steffen Thiel +8 more
TL;DR: Observations emphasize that MASP-3 acts as an important maturase in the AP of complement, while also highlighting that there exists MASp-3–independent pro-FD maturation in 3MC patients.
References
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Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome
Caroline Rooryck,Anna Diaz-Font,Daniel P. S. Osborn,Elyes Chabchoub,Victor Hernandez-Hernandez,Hanan E. Shamseldin,Joanna Kenny,Aoife M. Waters,Dagan Jenkins,Ali Al Kaissi,Gabriela F Leal,Bruno Dallapiccola,Franco Carnevale,Maria Bitner-Glindzicz,Melissa Lees,Raoul C.M. Hennekam,Philip Stanier,Alan J. Burns,Hilde Peeters,Fowzan S. Alkuraya,Fowzan S. Alkuraya,Philip L. Beales +21 more
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Effect of capsulation of opportunistic pathogenic bacteria on binding of the pattern recognition molecules mannan-binding lectin, L-ficolin, and H-ficolin.
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