Journal ArticleDOI
Liver extracellular matrix providing dual functions of two-dimensional substrate coating and three-dimensional injectable hydrogel platform for liver tissue engineering.
TLDR
LEM can provide functional biomaterial platforms for diverse applications in liver tissue engineering by promoting survival and maturation of hepatocytes and hepatic commitment of stem cells.About:
This article is published in Biomacromolecules.The article was published on 2014-01-13. It has received 200 citations till now. The article focuses on the topics: Decellularization & Tissue engineering.read more
Citations
More filters
Journal ArticleDOI
Extracellular matrix hydrogels from decellularized tissues: Structure and function.
Lindsey T. Saldin,Madeline C. Cramer,Sachin Velankar,Lisa J. White,Lisa J. White,Stephen F. Badylak +5 more
TL;DR: The present manuscript represents a review of ECM hydrogels and attempts to identify structure-function relationships that influence the tissue remodeling outcomes and gaps in the understanding thereof.
Journal ArticleDOI
Extracellular matrix-based materials for regenerative medicine
TL;DR: Challenges and opportunities of ECM biomaterials are investigated for the design of organotypic models to study disease progression, for the ex vivo creation of engineered tissue and for the clinical translation of functional tissue reconstruction strategies in vivo.
Journal ArticleDOI
Extracellular matrix hydrogel therapies: In vivo applications and development.
TL;DR: This review will focus on in vivo applications of ECM hydrogels and functional outcomes in disease models, as well as discuss considerations for clinical translation.
Journal ArticleDOI
Decellularized Extracellular Matrix-based Bioinks for Engineering Tissue- and Organ-specific Microenvironments.
TL;DR: This review aims to discuss a new paradigm of dECM-based bioinks able to recapitulate the inherent microenvironmental niche in 3D cell-printed constructs and a basic set of fundamental criteria for printing functional human tissues and organs.
Journal ArticleDOI
Organ-Derived Decellularized Extracellular Matrix: A Game Changer for Bioink Manufacturing?
TL;DR: It is emphasized that obtaining a good-quality bioink depends on the choice of organ, animal, and decellularization method, and potential large-scale applications of bioinks and challenges in manufacturing such bioinks are explored.
References
More filters
Book ChapterDOI
Preparation of isolated rat liver cells.
TL;DR: This chapter discusses preparation of isolated rat liver cells by incubation of rat liver minces with pronase, which results in most of the liver parenchyma is digested, while nonparenchymal cells remain intact and can be recovered from the incubate.
Journal ArticleDOI
Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue
TL;DR: A modified form of the dim methylmethylene blue assay is described that has improved specificity for sulphated glycosaminoglycans, and it is shown that in conjunction with specific polysaccharidases, the dimethylmethyleneblue assay can be used to quantitate individual sulphated sugarcans.
Journal ArticleDOI
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
Journal ArticleDOI
Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
Basak E. Uygun,Alejandro Soto-Gutierrez,Alejandro Soto-Gutierrez,Hiroshi Yagi,Hiroshi Yagi,Maria Louisa Izamis,Maria Angela Guzzardi,Maria Angela Guzzardi,Carley Shulman,Jack M. Milwid,Naoya Kobayashi,Arno W. Tilles,Francois Berthiaume,Francois Berthiaume,Martin Hertl,Yaakov Nahmias,Yaakov Nahmias,Martin L. Yarmush,Martin L. Yarmush,Korkut Uygun +19 more
TL;DR: A novel approach to generate transplantable liver grafts using decellularized liver matrix that preserves the structural and functional characteristics of the native microvascular network, allowing efficient recellularization of the liver matrix with adult hepatocytes and subsequent perfusion for in vitro culture.
Journal ArticleDOI
Tissue-Engineered Lungs for in Vivo Implantation
Thomas Petersen,Elizabeth A. Calle,Liping Zhao,Eun Jung Lee,Liqiong Gui,Micha Sam Brickman Raredon,Kseniya Gavrilov,Tai Yi,Zhen W. Zhuang,Christopher K. Breuer,Erica L. Herzog,Laura E. Niklason +11 more
TL;DR: Treating lungs from adult rats using a procedure that removes cellular components but leaves behind a scaffold of extracellular matrix suggests that repopulation of lung matrix is a viable strategy for lung regeneration.
Related Papers (5)
An overview of tissue and whole organ decellularization processes.
Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
Basak E. Uygun,Alejandro Soto-Gutierrez,Alejandro Soto-Gutierrez,Hiroshi Yagi,Hiroshi Yagi,Maria Louisa Izamis,Maria Angela Guzzardi,Maria Angela Guzzardi,Carley Shulman,Jack M. Milwid,Naoya Kobayashi,Arno W. Tilles,Francois Berthiaume,Francois Berthiaume,Martin Hertl,Yaakov Nahmias,Yaakov Nahmias,Martin L. Yarmush,Martin L. Yarmush,Korkut Uygun +19 more