Journal ArticleDOI
Marker-free phase nanoscopy
Yann Cotte,M. Fatih Toy,Pascal Jourdain,Nicolas Pavillon,Daniel Boss,Pierre J. Magistretti,Pierre Marquet,Christian Depeursinge +7 more
TLDR
In this paper, a quasi-2 pi-holographic detection scheme and complex deconvolution was used to obtain a lateral resolution of 90 nm. But the results were limited to the case of living dendritic spines (neurones).Abstract:
We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2 pi-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coil), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).read more
Citations
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Proceedings ArticleDOI
Optical metrology based on coherent phase imaging: applications to 3D deformation measurements and vibrations analysis
TL;DR: In this paper, the authors describe recent advances in coherent imaging based on digital holography; especially they focus on the use of such an approach to investigate traveling acoustic waves in mechanical structures and to study mechanical properties of bio-materials.
Patent
Tomographic phase microscope
TL;DR: In this paper, a coherent light source (4) producing coherent light beam (7), a light beam guide system (6) comprising a beam splitter (14), a sample holder (18), and a sample illumination device (28) configured to direct the sample illumination beam through the sample and into a microscope objective (37).
Journal ArticleDOI
Reflectional quantitative phase-contrast microscopy (RQPCM) with annular epi-illumination.
TL;DR: In this article , the authors proposed a reflectional quantitative phase contrast microscopy based on annular epi-illumination of light-emitting diodes, where the unscattered wave from the sample is successively phase-retarded by a spatial light modulator, revealing the finer structure or three-dimensional tomography of reflective samples.
Journal ArticleDOI
Accurate automatic object 4D tracking in digital in-line holographic microscopy based on computationally rendered dark fields
Mikołaj Rogalski,José Ángel Picazo-Bueno,Julianna Winnik,Piotr Zdańkowski,Vicente Micó,Maciej Trusiak +5 more
TL;DR: The DarkTrack algorithm as mentioned in this paper uses deterministic computationally rendered high-contrast dark fields to solve the inverse problem of particle tracking in 3D holographic microscopy and is able to achieve state-of-the-art performance.
Journal ArticleDOI
Quantitative phase imaging using a combination of flat fielding and windowed Fourier filtering demodulated by graph cuts algorithm for screening opaque and transparent objects
TL;DR: In this article , a combination of flat fielding and windowed Fourier filtering is used to measure the phase of opaque and transparent objects using graph cuts algorithm, and the results show that quantitative phases of the objects being screened are precisely measured by the proposed method.
References
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Journal ArticleDOI
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
PatentDOI
Tomographic phase microscopy
TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.
Journal ArticleDOI
A Filtered Backpropagation Algorithm for Diffraction Tomography
TL;DR: The reconstruction algorithm is derived for parallel beam transmission computed tomography through two-dimensional structures in which diffraction of the insonifying beam must be taken into account and is applicable to diffraction tomography within either the first Born or Rytov approximations.