Journal ArticleDOI
Marker-free phase nanoscopy
Yann Cotte,M. Fatih Toy,Pascal Jourdain,Nicolas Pavillon,Daniel Boss,Pierre J. Magistretti,Pierre Marquet,Christian Depeursinge +7 more
TLDR
In this paper, a quasi-2 pi-holographic detection scheme and complex deconvolution was used to obtain a lateral resolution of 90 nm. But the results were limited to the case of living dendritic spines (neurones).Abstract:
We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2 pi-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coil), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).read more
Citations
More filters
Posted ContentDOI
Label-free identification of non-activated lymphocytes using three-dimensional refractive index tomography and machine learning
TL;DR: The approach enables label-free identification of three lymphocyte cell types (B, CD4+ T, and CD8+ T lymphocytes) with high specificity and sensitivity and will be a versatile tool for investigating the pathophysiological roles of lymphocytes in various diseases including cancers, autoimmune diseases, and virus infections.
Journal ArticleDOI
Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
Cédric Allier,Romaric Vincent,Fabrice Navarro,Mathilde Menneteau,Lamya Ghenim,Lamya Ghenim,Xavier Gidrol,Thomas Bordy,Lionel Hervé,Olivier Cioni,Sabine Bardin,Michel Bornens,Yves Usson,Sophie Morales +13 more
TL;DR: It is demonstrated that lens-free video microscopy enables us to simultaneously capture the kinetics of thousands of cells directly inside the incubator and that it is possible to monitor and quantify single cells along several cell cycles.
Posted Content
Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes
TL;DR: An optical method based on 3-D quantitative phase imaging is presented to measure the3-D structural distribution and biochemical parameters (concentration and dry mass) of individual LDs in live cells without using exogenous labelling agents.
Proceedings ArticleDOI
Off-axis illumination in object-rotation diffraction tomography for enhanced alignment and resolution
TL;DR: In this article, an autofocusing method was proposed to solve the resolution limit and the rotation axis misalignment problem in optical diffraction tomography (ODT) by using simultaneous illumination from two fixed, highly off-axis directions.
Journal ArticleDOI
Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells
TL;DR: In this paper , a combination of confocal and wide-field interferometric scattering (iSCAT) modalities for simultaneous imaging of cellular structures and high-speed tracking of nanoscopic entities such as single SARS-CoV-2 virions is presented.
References
More filters
Journal ArticleDOI
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
PatentDOI
Tomographic phase microscopy
TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.
Journal ArticleDOI
A Filtered Backpropagation Algorithm for Diffraction Tomography
TL;DR: The reconstruction algorithm is derived for parallel beam transmission computed tomography through two-dimensional structures in which diffraction of the insonifying beam must be taken into account and is applicable to diffraction tomography within either the first Born or Rytov approximations.