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Journal ArticleDOI

Marker-free phase nanoscopy

TLDR
In this paper, a quasi-2 pi-holographic detection scheme and complex deconvolution was used to obtain a lateral resolution of 90 nm. But the results were limited to the case of living dendritic spines (neurones).
Abstract
We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2 pi-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coil), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).

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Citations
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Journal ArticleDOI

Aberration-free synthetic aperture phase microscopy based on alternating direction method

TL;DR: In this paper , an effective and straightforward global phase aberration-free synthetic-aperture phase microscopy (AF-SAPM) method was proposed for quantitative monitoring of endocrine tumor with highly variable phases.
Proceedings ArticleDOI

Characterizing spatio-temporal phase variation of pixelated liquid crystal on silicon using digital holographic microscopy

TL;DR: In this article, a spatio-temporal measurement technique for observing the phase variation of the LCoS devices using digital holographic microscopy is proposed, where the spatial distribution of phase variation in pixelated field was measured and characterized in millisecond scale.
Proceedings ArticleDOI

Moiré effect-based interference microscopy for biospecimen characterization

TL;DR: In this paper, two scenarios of incoherent multiplicative superimposition are employed: (1) object information carrying interferogram is superimposed with reference one or (2) two object interferograms are superimposed (with equal or opposite phase signs).
Proceedings ArticleDOI

Multi-beam spatially multiplexed interference microscopy for phase objects examination

TL;DR: Multi-beam Spatially Multiplexed Interferometric Microscopy (MB-SMIM) as discussed by the authors is a single-shot phase estimation algorithm with a simple, low-cost and stable way to convert a bright-field microscope into a holographic one using partially coherent illumination.
Journal ArticleDOI

Singular value demodulation of phase-shifted holograms

TL;DR: In this paper, phase-shifted holographic interferogram demodulation by singular value decomposition is described, and the results in low-light recording conditions are compared with demodulations by Fourier analysis, in the presence of random phase drifts.
References
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Journal ArticleDOI

Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.

TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI

Far-Field Optical Nanoscopy

TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI

Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms

TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
PatentDOI

Tomographic phase microscopy

TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.
Journal ArticleDOI

A Filtered Backpropagation Algorithm for Diffraction Tomography

A. J. Devaney
- 01 Oct 1982 - 
TL;DR: The reconstruction algorithm is derived for parallel beam transmission computed tomography through two-dimensional structures in which diffraction of the insonifying beam must be taken into account and is applicable to diffraction tomography within either the first Born or Rytov approximations.
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