Journal ArticleDOI
Marker-free phase nanoscopy
Yann Cotte,M. Fatih Toy,Pascal Jourdain,Nicolas Pavillon,Daniel Boss,Pierre J. Magistretti,Pierre Marquet,Christian Depeursinge +7 more
TLDR
In this paper, a quasi-2 pi-holographic detection scheme and complex deconvolution was used to obtain a lateral resolution of 90 nm. But the results were limited to the case of living dendritic spines (neurones).Abstract:
We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2 pi-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coil), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).read more
Citations
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Journal ArticleDOI
Enhancing spatial resolution in digital holographic microscopy by biprism structured illumination
TL;DR: The proposed method requires only two images to improve the spatial resolution, which eases the process of unmixing the high-resolution components by means of an unknown phase-shift procedure, and numerical modeling and experimental results validate the theoretical findings.
Journal ArticleDOI
Reconstruction method for extended depth-of-field optical diffraction tomography
TL;DR: A novel method of extended depth-of-field limited-angle optical diffraction tomography, in which the change of a focal plane position is performed with a liquid focus-tunable lens, which effectively extends the applicability of the Rytov approximation to relatively thick samples and provides uniform resolution of 3D tomographic reconstructions.
Posted Content
Optical diffraction tomography techniques for the study of cell pathophysiology
TL;DR: In this article, the authors present a summary of the optical diffraction tomography (ODT) principle and highlight recent studies utilizing ODT with the emphasis on the applications to the pathophysiology of cells.
Journal ArticleDOI
In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy
Martina Mugnano,Pasquale Memmolo,Lisa Miccio,Simonetta Grilli,Francesco Merola,Alejandro Calabuig,Alessia Bramanti,Emanuela Mazzon,Pietro Ferraro +8 more
TL;DR: The results show that the label‐free character of the technique would allow biologists to perform systematic and reliable studies on cell death process induced by cadmium and it is believed that more in general this can be easily extended to others heavy metals, thus avoiding the time‐consuming, expensive and invasive label‐based procedures used nowadays in the field.
Journal ArticleDOI
Mapping optical path length and image enhancement using quantitative orientation-independent differential interference contrast microscopy.
TL;DR: The principles of using orientation-independent differential interference contrast (OI-DIC) microscopy for mapping optical path length (OPL) are described and two methods of contrast enhancement for the OPL image, which reveal hardly visible structures and organelles are presented.
References
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Journal ArticleDOI
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TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
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TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
PatentDOI
Tomographic phase microscopy
TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.
Journal ArticleDOI
A Filtered Backpropagation Algorithm for Diffraction Tomography
TL;DR: The reconstruction algorithm is derived for parallel beam transmission computed tomography through two-dimensional structures in which diffraction of the insonifying beam must be taken into account and is applicable to diffraction tomography within either the first Born or Rytov approximations.