Journal ArticleDOI
Marker-free phase nanoscopy
Yann Cotte,M. Fatih Toy,Pascal Jourdain,Nicolas Pavillon,Daniel Boss,Pierre J. Magistretti,Pierre Marquet,Christian Depeursinge +7 more
TLDR
In this paper, a quasi-2 pi-holographic detection scheme and complex deconvolution was used to obtain a lateral resolution of 90 nm. But the results were limited to the case of living dendritic spines (neurones).Abstract:
We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2 pi-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coil), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).read more
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Posted Content
Diffraction tomography with Fourier ptychography
Roarke Horstmeyer,Changhuei Yang +1 more
TL;DR: In this article, a ptychography-based reconstruction algorithm solves for the unknown complex index of refraction across three dimensions in a standard microscope with an LED array for illumination, and the 3D tomograms are continuous, quantitative, and formed without the need for interferometry or any moving parts.
Journal ArticleDOI
Tomographic phase microscopy: principles and applications in bioimaging [Invited].
TL;DR: Tomographic phase microscopy (TPM) is an emerging optical microscopic technique for bioimaging as mentioned in this paper, which uses digital holographic measurements of complex scattered fields to reconstruct three-dimensional refractive index (RI) maps of cells with diffraction-limited resolution by solving inverse scattering problems.
Journal ArticleDOI
Digital holography and its multidimensional imaging applications: a review.
TL;DR: Digital holography can be used to perform multidimensional imaging of three-dimensional structure, dynamics, quantitative phase, multiple wavelengths, and polarization state of light and sensing of a holographic image of nonlinear light and a three- dimensional image of incoherent light.
Journal ArticleDOI
Recent Advances in the Analysis of Single Cells
Journal ArticleDOI
Quasi noise-free digital holography.
Vittorio Bianco,Pasquale Memmolo,Melania Paturzo,Andrea Finizio,Bahram Javidi,Pietro Ferraro +5 more
TL;DR: A novel framework is proposed that combines the concepts of encoding multiple uncorrelated digital holograms, block grouping and collaborative filtering to achieve quasi noise-free DH reconstructions and is comparable to the quality achievable with non-coherent techniques and far beyond the current state of art in DH.
References
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Journal ArticleDOI
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
PatentDOI
Tomographic phase microscopy
TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.
Journal ArticleDOI
A Filtered Backpropagation Algorithm for Diffraction Tomography
TL;DR: The reconstruction algorithm is derived for parallel beam transmission computed tomography through two-dimensional structures in which diffraction of the insonifying beam must be taken into account and is applicable to diffraction tomography within either the first Born or Rytov approximations.