Journal ArticleDOI
Marker-free phase nanoscopy
Yann Cotte,M. Fatih Toy,Pascal Jourdain,Nicolas Pavillon,Daniel Boss,Pierre J. Magistretti,Pierre Marquet,Christian Depeursinge +7 more
TLDR
In this paper, a quasi-2 pi-holographic detection scheme and complex deconvolution was used to obtain a lateral resolution of 90 nm. But the results were limited to the case of living dendritic spines (neurones).Abstract:
We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2 pi-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coil), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).read more
Citations
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Journal ArticleDOI
High Performance, Biocompatible Dielectric Thin‐Film Optical Filters Integrated with Flexible Substrates and Microscale Optoelectronic Devices
Changbo Liu,Qianyi Zhang,Dan Wang,Guanlei Zhao,Xue Cai,Lizhu Li,He Ding,Kaiyuan Zhang,Huachun Wang,Deying Kong,Lan Yin,Lei Liu,Guisheng Zou,Lingyun Zhao,Xing Sheng +14 more
Journal ArticleDOI
Resolution enhancement of spectrum normalization in synthetic aperture digital holographic microscopy.
TL;DR: The results indicated that using the normalized spectrum synthesis that requires only a few frequency bands effectively enhances the spatial resolution and phase sensitivity of reconstructed images in digital holographic microscopy.
Journal ArticleDOI
Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy.
Vijay Raj Singh,Vijay Raj Singh,Yi An Yang,Hanry Yu,Roger D. Kamm,Roger D. Kamm,Zahid Yaqoob,Peter T. C. So +7 more
TL;DR: In this article, a depth-resolved confocal reflectance interferometric microscopy (QPM) was proposed to study nuclear and plasma membrane biomechanics of complex eukaryotic cells.
Journal ArticleDOI
Compensated Microsphere-Assisted Interference Microscopy
TL;DR: In this article, the authors proposed an experimental method in microsphere-assisted interference microscopy that makes it possible to reconstruct surface topographies as much with high quantitative depth accuracy as when the transversal feature sizes are smaller than the classical diffraction limit.
Journal ArticleDOI
Functional imaging for regenerative medicine
Martin J. Leahy,Kerry Thompson,Haroon Zafar,Sergey A. Alexandrov,Mark Foley,Cathal O'Flatharta,Peter Dockery +6 more
TL;DR: The techniques that have found success in aiding discovery in stem cell therapies are explored and the likely technologies best suited to translation and future directions are predicted.
References
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Journal ArticleDOI
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
PatentDOI
Tomographic phase microscopy
TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.
Journal ArticleDOI
A Filtered Backpropagation Algorithm for Diffraction Tomography
TL;DR: The reconstruction algorithm is derived for parallel beam transmission computed tomography through two-dimensional structures in which diffraction of the insonifying beam must be taken into account and is applicable to diffraction tomography within either the first Born or Rytov approximations.