Journal ArticleDOI
Marker-free phase nanoscopy
Yann Cotte,M. Fatih Toy,Pascal Jourdain,Nicolas Pavillon,Daniel Boss,Pierre J. Magistretti,Pierre Marquet,Christian Depeursinge +7 more
TLDR
In this paper, a quasi-2 pi-holographic detection scheme and complex deconvolution was used to obtain a lateral resolution of 90 nm. But the results were limited to the case of living dendritic spines (neurones).Abstract:
We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2 pi-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coil), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).read more
Citations
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Journal ArticleDOI
Large field‐of‐view holographic imager with ultra‐high phase sensitivity using multi‐angle illumination
Sebastian Haegele,Giacomo Corrielli,Matěj Hejda,Luc Duempelmann,Roland A. Terborg,Roberto Osellame,Valerio Pruneri +6 more
TL;DR: In this article , a lens-free quantitative phase imaging for transparent materials is proposed and demonstrated with phase-shifting interferometry and multi-angle illumination that enhances axial resolution and image quality, enabling ultra-high phase sensitivity with optical path difference (OPD ) background root-mean-square (RMS) noise of 0.2 nm.
Proceedings ArticleDOI
Redundant Haar wavelet regularization in sparse-view optical diffraction tomography of microbiological structures
TL;DR: An iterative algorithm for sparse-angle optical diffraction tomography in 2D is proposed, which combines elements of the gridding reconstruction technique with the classical scheme of Fourier data consistency iterations subject to non-negativity constraint, additionally supplemented with gradient-domain and wavelet-domain sparsity constraints.
Journal ArticleDOI
Real-time measurement of three-dimensional morphology of blood cells in batches by non-orthogonal phase imaging
TL;DR: In this article, an optimized maximum entropy tomography (MET) algorithm is used for rapid 3D reconstruction which requires less phase information from non-orthogonal directions, and two phase images can be obtained simultaneously by the phase imaging system combined with flow cytometry and optical tweezers (OT).
Journal ArticleDOI
Coherent confocal light scattering spectroscopic microscopy evaluates cancer progression and aggressiveness in live cells and tissue
Douglas K. Pleskow,Lei Zhang,Vladimir Turzhitsky,Mark F. Coughlan,Umar Khan,Xuejun Zhang,Conor J. Sheil,Maria Glyavina,Liming Chen,Shweta Shinagare,Yuri Zakharov,Edward Vitkin,Irving Itzkan,Lev T. Perelman,Lev T. Perelman,Le Qiu +15 more
TL;DR: In this article, a coherent confocal light scattering and absorption spectroscopic microscopy was proposed to characterize structures in live cells down to 10 nm spatial scales, well beyond the diffraction limit.
Journal ArticleDOI
Versatile optimization-based speed-up method for autofocusing in digital holographic microscopy
TL;DR: In this paper, the authors proposed a speedup method for the in-focus plane detection in digital holographic microscopy that can be applied to a broad class of autofocusing algorithms that involve repetitive propagation of an object wave to various axial locations to decide the infocus position.
References
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Tomographic phase microscopy
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Journal ArticleDOI
A Filtered Backpropagation Algorithm for Diffraction Tomography
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