Journal ArticleDOI
Marker-free phase nanoscopy
Yann Cotte,M. Fatih Toy,Pascal Jourdain,Nicolas Pavillon,Daniel Boss,Pierre J. Magistretti,Pierre Marquet,Christian Depeursinge +7 more
TLDR
In this paper, a quasi-2 pi-holographic detection scheme and complex deconvolution was used to obtain a lateral resolution of 90 nm. But the results were limited to the case of living dendritic spines (neurones).Abstract:
We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2 pi-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coil), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).read more
Citations
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Journal ArticleDOI
Particle tracking by full-field complex wavefront subtraction in digital holography microscopy.
Lisa Miccio,Pasquale Memmolo,Francesco Merola,Sabato Fusco,Valerio Embrione,Antonio Paciello,Maurizio Ventre,Maurizio Ventre,Paolo A. Netti,Paolo A. Netti,Pietro Ferraro +10 more
TL;DR: The 3D tracking of micro-objects, based on digital holography, is proposed through the analysis of the complex wavefront of the light scattered by the micro-samples through a direct wavefront analysis at the focal plane overcoming the limitation of the conventional back focal plane interferometry.
Journal ArticleDOI
Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome
Dashan Dong,Xiaoshuai Huang,Liuju Li,Heng Mao,Yanquan Mo,Zhang Guangyi,Zhe Zhang,Jiayu Shen,Wei Liu,Wei Liu,Zeming Wu,Guang-Hui Liu,Yanmei Liu,Yanmei Liu,Hong Yang,Hong Yang,Qihuang Gong,Qihuang Gong,Kebin Shi,Kebin Shi,Liangyi Chen,Liangyi Chen +21 more
TL;DR: A team of Chinese researchers led by Liangyi Chen and Kebin Shi has created a novel dual-mode high-speed SR microscopy technique capable of revealing previously unseen sub-cellular structures and dynamic processes, allowing both the cellular landscape and molecular identity in live cells to be observed in 3D over prolonged periods.
Journal ArticleDOI
Rapid quantitative phase imaging for partially coherent light microscopy
José A. Rodrigo,Tatiana Alieva +1 more
TL;DR: A non-interferometric technique and system for quantitative phase imaging with simultaneous determination of the spatial coherence properties of the sample illumination and its performance is experimentally demonstrated underlining the benefits of partial coherence for practical imagining applications.
Journal ArticleDOI
Generalized total variation iterative constraint strategy in limited angle optical diffraction tomography.
TL;DR: A new two-stage regularization strategy, named Generalized Total Variation Iterative Constraint (GTVIC), dedicated to semi-piecewise-constant objects, successfully applied as a supplementary module for two different reconstruction algorithms: an X-ray type solver and a diffraction-wise solver.
Journal ArticleDOI
Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning.
TL;DR: The noninvasive, fast acquisition of quantitative phase maps using digital holographic microscopy (DHM) allows tracking of rapid cellular motility on transparent substrates and indicates the potential of DHM to detect and monitor cancer cell morphologies relevant to cell cycle phase status, substrate adhesion, and motility.
References
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Journal ArticleDOI
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
PatentDOI
Tomographic phase microscopy
TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.
Journal ArticleDOI
A Filtered Backpropagation Algorithm for Diffraction Tomography
TL;DR: The reconstruction algorithm is derived for parallel beam transmission computed tomography through two-dimensional structures in which diffraction of the insonifying beam must be taken into account and is applicable to diffraction tomography within either the first Born or Rytov approximations.