Mass and stiffness spectrometry of nanoparticles and whole intact bacteria by multimode nanomechanical resonators.
Óscar Malvar,J. J. Ruz,Priscila M. Kosaka,Carmen Domínguez,Eduardo Gil-Santos,Montserrat Calleja,Javier Tamayo +6 more
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TLDR
By using nanomechanical resonators, heavier analytes can be identified by their mass and stiffness by demonstrating the enormous potential of this technology for identification of large biological complexes near their native conformation.Abstract:
Mass spectrometry can accurately identify species by molecular mass, but measuring large species can be difficult. Here the authors show that nanomechanical resonators can identify both the mass and stiffness of larger analytes, demonstrating it for gold nanoparticles and E. Coli bacteria.read more
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The emerging landscape of single-molecule protein sequencing technologies
Javier A. Alfaro,Peggy R. Bohländer,Mingjie Dai,Mike Filius,Cecil J Howard,Xander F. van Kooten,Shilo Ohayon,Adam Pomorski,Sonja Schmid,Aleksei Aksimentiev,Eric V. Anslyn,Georges Bedran,Chan Cao,Mauro Chinappi,Etienne Coyaud,Cees Dekker,Gunnar Dittmar,Nicholas Drachman,Rienk Eelkema,David R. Goodlett,David R. Goodlett,Sebastien Hentz,Umesh Kalathiya,Neil L. Kelleher,Ryan T. Kelly,Zvi Kelman,Sung Hyun Kim,Bernhard Kuster,David Rodriguez-Larrea,Stuart Lindsay,Giovanni Maglia,Edward M. Marcotte,John P. Marino,Christophe Masselon,Michael Mayer,Patroklos Samaras,Kumar Sarthak,Lusia Sepiashvili,Derek Stein,Meni Wanunu,Mathias Wilhelm,Peng Yin,Amit Meller,Chirlmin Joo +43 more
TL;DR: In this paper, the authors describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling.
Journal ArticleDOI
Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators
Sergio Dominguez-Medina,Sergio Dominguez-Medina,Shawn Fostner,Martial Defoort,Marc Sansa,Ann-Kathrin Stark,Ann-Kathrin Stark,Mohammad A. Halim,Mohammad A. Halim,Emeline Vernhes,Marc Gely,Guillaume Jourdan,Thomas Alava,Pascale Boulanger,Christophe Masselon,Christophe Masselon,Sebastien Hentz +16 more
TL;DR: This system determined the mass distribution of ~30-megadalton polystyrene nanoparticles with high detection efficiency and effectively performed molecular mass measurements of empty or DNA-filled bacteriophage T5 capsids with masses up to 105 megadaltons using less than 1 picomole of sample and with an instrument resolution above 100.
Journal ArticleDOI
Optomechanical detection of vibration modes of a single bacterium.
Eduardo Gil-Santos,J. J. Ruz,Óscar Malvar,Ivan Favero,Aristide Lemaître,Priscila M. Kosaka,Sergio García-López,Montserrat Calleja,Javier Tamayo +8 more
TL;DR: This work harnessed a particular regime in the physics of coupled mechanical resonators to directly measure these low-frequency mechanical resonances of a single bacterium, demonstrating that ultrahigh frequency optomechanical resonators can be used for vibrational spectrometry with the unique capability to obtain information on single biological entities.
Journal ArticleDOI
Single-particle mass spectrometry with arrays of frequency-addressed nanomechanical resonators
Eric Sage,Marc Sansa,Shawn Fostner,Martial Defoort,Marc Gely,Akshay Naik,R. Morel,Laurent Duraffourg,Michael L. Roukes,Thomas Alava,Guillaume Jourdan,Eric Colinet,Christophe Masselon,Christophe Masselon,Ariel Brenac,Sebastien Hentz +15 more
TL;DR: The demonstration of mass spectrometry with arrays of 20 multiplexed nanomechanical resonators; each resonator is designed with a distinct resonance frequency which becomes its individual address, which significantly decreases detection time.
Journal ArticleDOI
Optomechanical mass spectrometry.
Marc Sansa,Martial Defoort,Ariel Brenac,Maxime Hermouet,Louise Banniard,Alexandre Fafin,Marc Gely,Christophe Masselon,Ivan Favero,Guillaume Jourdan,Sebastien Hentz +10 more
TL;DR: Single-particle mass spectrometry using integrated optomechanical resonators, impervious to particle position, stiffness or shape is demonstrated, showing a three-fold improvement in capture area with no resolution degradation, despite the use of a single resonance mode.
References
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TL;DR: The ability of mass spectrometry to identify and, increasingly, to precisely quantify thousands of proteins from complex samples can be expected to impact broadly on biology and medicine.
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Frequency response of cantilever beams immersed in viscous fluids with applications to the atomic force microscope
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Zeptogram-Scale Nanomechanical Mass Sensing
TL;DR: Analysis of the ultimate sensitivity of very high frequency nanoelectromechanical systems indicates that NEMS can ultimately provide inertial mass sensing of individual intact, electrically neutral macromolecules with single-Dalton (1 amu) resolution.
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A nanomechanical mass sensor with yoctogram resolution
TL;DR: This unprecedented level of sensitivity allows us to detect adsorption events of naphthalene molecules, and to measure the binding energy of a xenon atom on the nanotube surface, which could have applications in mass spectrometry, magnetometry and surface science.
Posted Content
Nanoelectromechanical Systems
TL;DR: Nanoelectromechanical systems as discussed by the authors are MEMS scaled to submicron dimensions, which can attain extremely high fundamental frequencies while simultaneously preserving very high mechanical responsivity (small force constants).