Members of the sp transcription factor family control transcription from the uteroglobin promoter
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TLDR
It is reported that members of the Sp transcription factor family specifically recognize this non-classical GC box, in addition to another functional motif located 230 base pairs upstream of the transcriptional start site.About:
This article is published in Journal of Biological Chemistry.The article was published on 1995-05-26 and is currently open access. It has received 109 citations till now. The article focuses on the topics: Promoter & Response element.read more
Citations
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Journal ArticleDOI
The Sp-family of transcription factors
TL;DR: Molecular, genetic and biochemical analyses demonstrated that Sp2, Sp3 and Sp4 are not simply functional equivalents of Sp1.
Book ChapterDOI
Transcriptional activation of genes by 17 beta-estradiol through estrogen receptor-Sp1 interactions.
TL;DR: Results of ongoing studies suggest that ER alpha/Sp1 plays an important role in transcriptional activation of multiple growth regulatory genes in breast cancer cells.
Journal ArticleDOI
Sp3 Is a Bifunctional Transcription Regulator with Modular Independent Activation and Repression Domains
TL;DR: It is demonstrated that Sp3 is a bifunctional transcription factor that can both activate and repress transcription, and the repression activity is strictly dependent on the context of the DNA-binding sites bound by Sp3.
Journal ArticleDOI
Transcriptional regulation by the Sp family proteins
TL;DR: This review focuses primarily on strategies aimed to elucidate the transcription properties of the Sp1-like factors and discusses the experimental problems inherent in the attempt to define their respective functions.
Journal ArticleDOI
Keep your fingers off my DNA: Protein-protein interactions mediated by C2H2 zinc finger domains
Kathryn J. Brayer,David J. Segal +1 more
TL;DR: The current knowledge of over 100 C2H2 zinc finger-mediated PPIs is reviewed, focusing on what is known about the binding surface, contributions of individual fingers to the interaction, and function.
References
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Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
TL;DR: A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.
Journal ArticleDOI
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis
Michael Fried,Donald M. Crothers +1 more
TL;DR: Gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites.
Journal ArticleDOI
A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells.
TL;DR: The aim in developing this micropreparation procedure was to easily and rapidly extract DNA-binding proteins from small numbers of cells, and it gives an excellent yield, comparable to that of the large scale Dignam protocol with minimal proteolysis.
Journal ArticleDOI
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
TL;DR: It is found that purified Sp1 requires Zn(II) for sequence-specific binding to DNA, and it is likely that Sp1 interacts with DNA by binding of the Zn (II) fingers.
Related Papers (5)
Sp1-mediated transcriptional activation is repressed by Sp3.
Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes
Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression.
C Kingsley,Astar Winoto +1 more