Journal ArticleDOI
Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways
Claire R. Shen,James C. Liao +1 more
TLDR
This work systematically improved the synthesis of 1-propanol and 1-butanol through deregulation of amino-acid biosynthesis and elimination of competing pathways.About:
This article is published in Metabolic Engineering.The article was published on 2008-11-01. It has received 408 citations till now. The article focuses on the topics: Norvaline & Metabolic engineering.read more
Citations
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Journal ArticleDOI
Alcohol combustion chemistry
TL;DR: A detailed overview of recent results on alcohol combustion can be found in this paper, with a particular emphasis on butanols and other linear and branched members of the alcohol family, from methanol to hexanols.
Journal ArticleDOI
Biofuel combustion chemistry: from ethanol to biodiesel.
Katharina Kohse-Höinghaus,Patrick Oßwald,Terrill A. Cool,Tina Kasper,Nils Hansen,Fei Qi,Charles K. Westbrook,Phillip R. Westmoreland +7 more
TL;DR: Some characteristic aspects of the chemical pathways in the combustion of prototypical representatives of potential biofuels are highlighted, which focus on the decomposition and oxidation mechanisms and the formation of undesired, harmful, or toxic emissions.
Journal ArticleDOI
Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals
TL;DR: The reversal of the β-oxidation cycle was engineered in Escherichia coli and used in combination with endogenous dehydrogenases and thioesterases to synthesize n-alcohols, fatty acids and 3-hydroxy-, 3-keto- and trans-Δ2-carboxylic acids.
Journal ArticleDOI
Developments in biobutanol production: New insights
Manish Kumar,Kalyan Gayen +1 more
TL;DR: Clostridium beijerinckii is being explored as promising strain to produce biobutanol from cellulosic materials and selection of suitable bacterial strain is focused on, as well as availability of cheaper biomass to produce butanol, metabolic engineering strategies of various microorganisms are focused on.
Journal ArticleDOI
Use of higher alcohol biofuels in diesel engines: A review
B. Rajesh Kumar,S. Saravanan +1 more
TL;DR: In this paper, the effects of using higher alcohols ranging from 3-carbon propanol to 20-carbon phytol on combustion, performance and emission characteristics of a wide range of diesel engines under various test conditions.
References
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.
Tomoya Baba,Takeshi Ara,Miki Hasegawa,Yuki Takai,Yoshiko Okumura,Miki Baba,Kirill A. Datsenko,Masaru Tomita,Barry L. Wanner,Hirotada Mori,Hirotada Mori +10 more
TL;DR: These mutants—the ‘Keio collection’—provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome‐wide testing of mutational effects in a common strain background, E. coli K‐12 BW25113.
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Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
TL;DR: Gene splicing by overlap extension is a new approach for recombining DNA molecules at precise junctions irrespective of nucleotide sequences at the recombination site and without the use of restriction endonucleases or ligase.
Journal ArticleDOI
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
TL;DR: This strategy uses the host’s highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis to achieve high-yield, high-specificity production of isobutanol from glucose.
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Independent and Tight Regulation of Transcriptional Units in Escherichia Coli Via the LacR/O, the TetR/O and AraC/I1-I2 Regulatory Elements
Rolf Lutz,Hermann Bujard +1 more
TL;DR: Controlling the expression of the genes encoding luciferase, the low abundance E.coli protein DnaJ and restriction endonuclease Cfr9I not only demonstrates that high levels of expression can be achieved but also suggests that under conditions of optimal repression only around one mRNA every 3rd generation is produced.