Metabolic features of chronic fatigue syndrome
Robert K. Naviaux,Jane C. Naviaux,Kefeng Li,A. Taylor Bright,William A. Alaynick,Lin Wang,Asha Baxter,Neil Nathan,Wayne F. Anderson,Eric M Gordon +9 more
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TLDR
Targeted, broad-spectrum metabolomics of plasma showed that chronic fatigue syndrome is a highly concerted hypometabolic response to environmental stress that traces to mitochondria and was similar to the classically studied developmental state of dauer, opening a fresh path for the rational development of new therapeutics.Abstract:
More than 2 million people in the United States have myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). We performed targeted, broad-spectrum metabolomics to gain insights into the biology of CFS. We studied a total of 84 subjects using these methods. Forty-five subjects (n = 22 men and 23 women) met diagnostic criteria for ME/CFS by Institute of Medicine, Canadian, and Fukuda criteria. Thirty-nine subjects (n = 18 men and 21 women) were age- and sex-matched normal controls. Males with CFS were 53 (±2.8) y old (mean ± SEM; range, 21–67 y). Females were 52 (±2.5) y old (range, 20–67 y). The Karnofsky performance scores were 62 (±3.2) for males and 54 (±3.3) for females. We targeted 612 metabolites in plasma from 63 biochemical pathways by hydrophilic interaction liquid chromatography, electrospray ionization, and tandem mass spectrometry in a single-injection method. Patients with CFS showed abnormalities in 20 metabolic pathways. Eighty percent of the diagnostic metabolites were decreased, consistent with a hypometabolic syndrome. Pathway abnormalities included sphingolipid, phospholipid, purine, cholesterol, microbiome, pyrroline-5-carboxylate, riboflavin, branch chain amino acid, peroxisomal, and mitochondrial metabolism. Area under the receiver operator characteristic curve analysis showed diagnostic accuracies of 94% [95% confidence interval (CI), 84–100%] in males using eight metabolites and 96% (95% CI, 86–100%) in females using 13 metabolites. Our data show that despite the heterogeneity of factors leading to CFS, the cellular metabolic response in patients was homogeneous, statistically robust, and chemically similar to the evolutionarily conserved persistence response to environmental stress known as dauer.read more
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Challenges and emergent solutions for LC-MS/MS based untargeted metabolomics in diseases.
Liang Cui,Haitao Lu,Yie Hou Lee +2 more
TL;DR: Given the rapidly growing utility of metabolomics, this review will offer new perspectives, increase awareness of the major challenges in LC-MS metabolomics that will significantly benefit the metabolomics community and also the broader the biomedical community metabolomics aspire to serve.
Journal ArticleDOI
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome - Evidence for an autoimmune disease
Franziska Sotzny,Julià Blanco,Enrica Capelli,Jesús Castro-Marrero,Sophie Steiner,Modra Murovska,Carmen Scheibenbogen,Cfs (Euromene) +7 more
TL;DR: Current autoimmune aspects for ME/CFS are discussed including changes in cytokine profiles and immunoglobulin levels, T- and B-cell phenotype and a decrease of natural killer cell cytotoxicity, and autoantibodies against various antigens including neurotransmitter receptors.
Journal ArticleDOI
Chronic viral infections in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS).
Santa Rasa,Zaiga Nora-Krukle,Nina Henning,Eva Eliassen,Evelina Shikova,Thomas Harrer,Carmen Scheibenbogen,Modra Murovska,Bhupesh K. Prusty +8 more
TL;DR: This review aims to compile all the studies done so far to investigate various viral agents that could be associated with ME/CFS and suggest strategies to better design future studies on the role of viral infections in this disease.
Journal ArticleDOI
Long COVID and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)-A Systemic Review and Comparison of Clinical Presentation and Symptomatology.
Timothy L. Wong,Danielle Weitzer +1 more
TL;DR: A systemic literature search was conducted in MEDLINE and PsycInfo through to 31 January 2021 for studies related to long COVID symptomatology as discussed by the authors, which defines a series of chronic symptoms that patients may experience after resolution of acute COVID-19.
Journal ArticleDOI
Fecal metagenomic profiles in subgroups of patients with myalgic encephalomyelitis/chronic fatigue syndrome
Dorottya Nagy-Szakal,Brent L. Williams,Nischay Mishra,Xiaoyu Che,Bohyun Lee,Lucinda Bateman,Nancy G. Klimas,Nancy G. Klimas,Anthony L. Komaroff,Susan Levine,Jose G. Montoya,Daniel L. Peterson,Devi Ramanan,Komal Jain,M L Eddy,Mady Hornig,W. Ian Lipkin +16 more
TL;DR: Findings indicate that dysbiotic features that are uniquely ME/CFS-associated may be masked by disturbances arising from the high prevalence of IBS co-morbidity in ME-CFS, and these insights may enable more accurate diagnosis and lead to insights that inform the development of specific therapeutic strategies in ME/ CFS subgroups.
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