Journal ArticleDOI
Methods for the determination of limit of detection and limit of quantitation of the analytical methods
TLDR
The presented review provides information relating to the calculation of the limit of detection and limit of quantitation.Abstract:
The quality of an analytical method developed is always appraised in terms of suitability for its intended purpose, recovery, requirement for standardization, sensitivity, analyte stability, ease of analysis, skill subset required, time and cost in that order. It is highly imperative to establish through a systematic process that the analytical method under question is acceptable for its intended purpose. Limit of detection (LOD) and limit of quantification (LOQ) are two important performance characteristics in method validation. LOD and LOQ are terms used to describe the smallest concentration of an analyte that can be reliably measured by an analytical procedure. There has often been a lack of agreement within the clinical laboratory field as to the terminology best suited to describe this parameter. Likewise, there have been various methods for estimating it. The presented review provides information relating to the calculation of the limit of detection and limit of quantitation. Brief information about differences in various regulatory agencies about these parameters is also presented here.read more
Citations
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Electrically-Transduced Chemical Sensors Based on Two-Dimensional Nanomaterials
TL;DR: Key advances in the application of 2D materials, from both a historical and analytical perspective, are summarized for four different groups of analytes: gases, volatile compounds, ions, and biomolecules.
Journal ArticleDOI
Optimization and validation of a quantitative liquid chromatography-tandem mass spectrometric method covering 295 bacterial and fungal metabolites including all regulated mycotoxins in four model food matrices.
TL;DR: It can be concluded that quantitative determination of mycotoxins by LC-MS/MS based on a "dilute and shoot" approach is also feasible in case of complex matrices.
Journal ArticleDOI
Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR).
TL;DR: This work presents methods to determine the limit of detection (LoD) and thelimit of quantification (LoQ) as applicable to qPCR, based on standard statistical methods as recommended by regulatory bodies adapted toqPCR and complemented with a novel approach to estimate the precision of LoD.
Journal ArticleDOI
Turn-off fluorescence sensor for the detection of ferric ion in water using green synthesized N-doped carbon dots and its bio-imaging.
Thomas Nesakumar Jebakumar Immanuel Edison,Raji Atchudan,Jae-Jin Shim,Senthilkumar Kalimuthu,Byeong-Cheol Ahn,Yong Rok Lee +5 more
TL;DR: The synthesized N-CDs were used as a fluorescent probe towards the selective and sensitive detection of biologically important Fe(3+) ions in water by fluorescence spectroscopy and for bio-imaging of MDA-MB-231 cells.
Journal ArticleDOI
Biological imaging without autofluorescence in the second near-infrared region
Shuo Diao,Guosong Hong,Alexander L. Antaris,Jeffrey L. Blackburn,Kai Cheng,Zhen Cheng,Hongjie Dai +6 more
TL;DR: It is reported that fluorescence imaging in the 1,500–1,700-nm region (termed “NIR-IIb”) under 808-nm excitation results in nearly zero tissue autofluorescence, allowing for background-free imaging of fluorescent species in otherwise notoriously aut ofluorescent biological tissues, including liver.
References
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Journal ArticleDOI
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TL;DR: In this article, the Working Group that produced these protocols/guidelines has now been mandated by IUPAC to prepare guidelines on the single-laboratory validation of methods of analysis, providing minimum recommendations on procedures that should be employed to ensure adequate validation of analytical methods.
Limit of blank, limit of detection and limit of quantitation.
David A Armbruster,Terry Pry +1 more
TL;DR: The Limit of Blank, Limit of Detection, and Limit of Quantitation are terms used to describe the smallest concentration of a measurand that can be reliably measured by an analytical procedure.
Journal Article
Comparison of various international guidelines for analytical method validation.
S Chandran,R S P Singh +1 more
TL;DR: Analytical method validation is the systematic process of establishing that an analytical method is acceptable for its intended purpose as mentioned in this paper, which has a direct impact on the quality of the above data, such validated analytical methods for qualitative or quantitative testing of drug molecules assume greater importance when they are employed to generate quality and safety compliance data during development and postapproval of drug products.
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Comparison of signal-to-noise, blank determination, and linear regression methods for the estimation of detection and quantification limits for volatile organic compounds by gas chromatography.
Mohd Marsin Sanagi,Susie L Ling,Zalilah Nasir,Dadan Hermawan,Wan Aini Wan Ibrahim,Ahmedy Abu Naim +5 more
TL;DR: This work compares three methods based on the International Conference on Harmonization and EURACHEM guidelines, namely, signal-to-noise, blank determination, and linear regression, to estimate the LOD and LOQ for volatile organic compounds (VOCs) by experimental methodology using GC.
Journal ArticleDOI
Determination of niacin in infant formula by solid-phase extraction/liquid chromatography: peer-verified method performance-interlaboratory validation.
TL;DR: Integration of the AOAC Peer-Verified Method process with a readily available certified reference material gives the user confidence in the accuracy of the data generated by the method through traceability to the reference material used.