Journal ArticleDOI
Molecular Beacon Aptamers for Protein Monitoring in Real-Time and in Homogeneous Solutions
TLDR
This work has shown that aptamers hold great potential for high throughput protein analysis in areas such as disease diagnosis and functional proteomics.Abstract:
Aptamers are nucleic acids selected for binding target molecules of interest with high affinity and selectivity. They have seen increasing application in protein detection due to many of their advantages over traditional protein probes such as antibodies. Aptamers' robust yet flexible functional structures and relatively small sizes have allowed us to develop several strategies for sensitive protein detection in real time and in homogeneous solutions while posing minimum effects on the biological activities of the proteins. Quantitative protein analyses were done using aptamers labeled with a fluorophore and a quencher based on fluorescence resonance energy transfer (FRET), or using aptamers labeled with only one fluorophore based on fluorescence anisotropy. Real world biological samples were tested for the presence of target proteins. We believe that aptamers hold great potential for high throughput protein analysis in areas such as disease diagnosis and functional proteomics.read more
Citations
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Journal ArticleDOI
DNA-Mediated Homogeneous Binding Assays for Nucleic Acids and Proteins
Journal ArticleDOI
Abasic Site-Containing DNAzyme and Aptamer for Label-Free Fluorescent Detection of Pb2+ and Adenosine with High Sensitivity, Selectivity, and Tunable Dynamic Range
Yu Xiang,Aijun Tong,Yi Lu +2 more
TL;DR: The method demonstrated here can be applied for label-free detection and quantification of a broad range of analytes using other DNAzymes and aptamers, and the dynamic range of the adenosine sensor has been tuned by varying the number of hybridized base-pairs in the aptamer duplex.
Journal ArticleDOI
Aptamers as molecular recognition elements for electrical nanobiosensors.
TL;DR: This review summarizes the nanoscale biosensors that use aptamers as molecular recognition elements and the advantages of aptamer over antibodies as sensors are highlighted.
Journal ArticleDOI
Recent Development of Sandwich Assay Based on the Nanobiotechnologies for Proteins, Nucleic Acids, Small Molecules, and Ions
TL;DR: Nanobiotechnologies for Proteins, Nucleic Acids, Small Molecules, and Ions are presented.
References
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Journal ArticleDOI
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Craig Tuerk,Larry Gold +1 more
TL;DR: High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species.
Journal ArticleDOI
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Journal ArticleDOI
Electrospray ionization for mass spectrometry of large biomolecules
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Journal ArticleDOI
Mass spectrometry-based proteomics
Ruedi Aebersold,Matthias Mann +1 more
TL;DR: The ability of mass spectrometry to identify and, increasingly, to precisely quantify thousands of proteins from complex samples can be expected to impact broadly on biology and medicine.
Journal ArticleDOI
Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons
Michael Karas,Franz Hillenkamp +1 more
TL;DR: In the following, the first results on ultraviolet laser desorption (UVLD) of bioorganic compounds in the mass range above 10000 daltons are reported.
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