Journal ArticleDOI
Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation.
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TLDR
A novel signaling mechanism in which thrombin cleaves its receptor's amino-terminal extension to create a new receptor amino terminus that functions as a tethered ligand and activates the receptor is revealed.About:
This article is published in Cell.The article was published on 1991-03-22. It has received 2992 citations till now. The article focuses on the topics: Thrombin receptor & Protease-Activated Receptor 1.read more
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Thrombin signalling and protease-activated receptors
TL;DR: Roles for PARs are beginning to emerge in haemostasis and thrombosis, inflammation, and perhaps even blood vessel development.
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An analysis of vertebrate mRNA sequences: intimations of translational control.
TL;DR: In this paper, structural features in mRNAs have been found to contribute to the fidelity and efficiency of initiation by eukaryotic ribosomes, and it was suggested that throttling at the level of translation may be a critical component of gene regulation in vertebrates.
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G Protein-coupled Receptors I. DIVERSITY OF RECEPTOR-LIGAND INTERACTIONS
TL;DR: The most striking difference has been observed in the sites and modes of ligand binding and signal generation, which not only manifests the diversity but also indicates the availability of numerous alternative approaches to clinical and industrial applications.
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Signaling Mechanisms Regulating Endothelial Permeability
Dolly Mehta,Asrar B. Malik +1 more
TL;DR: This review summarizes and analyzes the recent data from genetic, physiological, cellular, and morphological studies that have addressed the signaling mechanisms involved in the regulation of both the paracellular and transcellular transport pathways.
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Mechanisms of Thrombus Formation
Bruce Furie,Barbara C. Furie +1 more
TL;DR: This review is an account of recent advances in the understanding of the mechanisms of thrombus formation, with emphasis on two independent pathways: one involving primarily platelets and the other initiated by tissue factor.
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
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A new generation of Ca2+ indicators with greatly improved fluorescence properties.
TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.
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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
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Rapid and efficient site-specific mutagenesis without phenotypic selection.
TL;DR: The high efficiency, approximately equal to 10-fold greater than that observed using current methods without enrichment procedures, is obtained by using a DNA template containing several uracil residues in place of thymine, which is applied to mutations introduced via both oligonucleotides and error-prone polymerization.