Journal ArticleDOI
Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor strains isolated before, during and after the O139 outbreak based on the intergenomic heterogeneity of the 16S-23S rRNA intergenic spacer regions
TLDR
Phylogenetic analysis conducted with all three ISR classes showed that the pre-O 139 serogroup and post-O139 serogroups O1 El Tor strains arose out of two independent clones, which was congruent with the observation made by earlier workers suggesting that analyses of ISR-C andISR-h, instead of all fiveISR classes, could be successfully used to study phylogeny in this organism.Abstract:
We have cloned, sequenced and analysed all the five classes of the intergenic (16S-23S rRNA) spacer region (ISR) associated with the eightrrn operons (rrna-rrnh) ofVibrio cholerae serogroup O1 El Tor strains isolated before, during and after the O139 outbreak. ISR classes ‘a’ and ‘g’ were found to be invariant, ISR-B (ISRb and ISRe) exhibited very little variation, whereas ISR-C (ISRc, ISRd, and ISRf) and ISRh showed the maximum variation. Phylogenetic analysis conducted with all three ISR classes (ISR-B, ISR-C and ISRh) showed that the pre-O139 serogroup and post-O139 serogroup O1 El Tor strains arose out of two independent clones, which was congruent with the observation made by earlier workers suggesting that analyses of ISR-C and ISR-h, instead of all five ISR classes, could be successfully used to study phylogeny in this organism.read more
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The use of molecular techniques to characterize the microbial communities in contaminated soil and water
TL;DR: This review examines the current application of molecular techniques for the characterization of microbial communities in contaminated soil and water and methods that directly link microbial phylogeny to its ecological function at contaminated sites as well as high throughput methods for complex microbial community studies.
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Characterization of potentially virulent non-O1/non-O139 Vibrio cholerae strains isolated from human patients
F.A.M.O. Cariri,Ana Paula Rocha da Costa,C.C. Melo,Grace Nazareth Diogo Theophilo,Ernesto Hofer,O.P. de Melo Neto,Nilma Cintra Leal +6 more
TL;DR: In this paper, the presence of the O1-specific wbeN gene was investigated through PCR and found to be restricted to strain 17155, as well as one of O26 strains, 4756, although neither strain was recognized by O1specific antisera.
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T. Ramamurthy,Surabhi Garg,R. B. Sharma,S. K. Bhattacharya,G. Balakrish Nair,Toshio Shimada,Tae Takeda,Tadahiro Karasawa,Hisao Kurazano,Amit Pal +9 more
Advances in Molecular Biology Approaches to Guage Microbial Communities and Bioremediation at Contaminated Sites
TL;DR: The potential for the use of molecular methods in toxicological risk assessment and in developing bioremediation strategies is expanding rapidly as new methodologies become available.
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Application of Molecular Microbiology Techniques in Bioremediation of Hydrocarbons and Other Pollutants
TL;DR: The application of molecular microbiology techniques Studying microbial populations in polluted sites without the need for culturing has led to the discovery of novel and unrecog nized populations.
References
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Phylogeny of Porphyromonas gingivalis by ribosomal intergenic spacer region analysis.
TL;DR: The phylogeny of P. gingivalis was constructed on the basis of the sequence of the most variable region of the ribosomal operon, the intergenic spacer region (ISR) and close agreement was observed, suggesting a relationship between pathogenicity and phylogeny.
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Über Cholera- und choleraähnliche Vibrionen unter den aus Mekka zurückkehrenden Pilgern: Ein Beitrag zur Epidemiologie der Cholera
TL;DR: Das Kampement yon Tor, an der Westkiiste der Sinaihalbinsel gelegen, hat bekanntlich die hufgalse, die Seuchengefahr, welche allj~ihrlieh Egypten and den Mittelmeerl~ndern aus zur Zeit der Pilgerfahrt droht, dureh entsprechende Quarant~nemal~regeln abzuw
Journal ArticleDOI
Studies on the genesis of Vibrio cholerae 0139: Identification of probable progenitor strains
Sangeeta Pajni,Charu Sharma,Navneet Bhasin,Amit Ghosh,T. Ramamurthy,G. Balakrish Nair,S. Ramajayam,Biswajit Das,Sudeshna Kar,Saumya Roychowdhury,R. K. Ghosh +10 more
TL;DR: Four lines of evidence suggest that the recent outbreak strains of Vibrio cholerae O139 could have emerged from serogroup O1 strains typified by isolates M01 and M0477, which are neither truly classical nor truly E1 Tor in their biotype attributes.