Journal ArticleDOI
Morphology and cellular-traction of fibroblasts on 2D silk-fibroin hydrogel substrates
TLDR
Results suggest that surface-stiffness of SF-hydrogel, rather than nature of surface-ligand, regulates both cellular morphology and cellular traction stresses.Abstract:
Development of clinically amenable bio-implants with silk-fibroin (SF) necessitates characterization of cellular-traction generated between cells and the substrate. However, studies on the biomecha...read more
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Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications
TL;DR: Wang et al. as mentioned in this paper describe varied building blocks of silk at different levels used in biomedical field and their effective extraction and reconstruction strategies, and present recent discoveries and research progresses on how these functional regenerated silk fibroin (RSF) biomaterials used in advanced biomedical applications, especially in the fields of cell-material interactions, soft tissue regeneration, and flexible bioelectronic devices.
Journal ArticleDOI
Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review
Guoqing Zhao,Md Abdul Goni +1 more
TL;DR: A review of the effects of mechanosignalling in the field of cellular mechanobiology can be found in this paper , where the authors discuss some of the interesting works wherein specific alteration of the mechanical properties of the substrates would lead to fate determination of stem cells into various differentiated cells such as osteoblasts, adipocytes, tenocytes, cardiomyocytes, and neurons, and how these properties are being utilized for the development of organoids.
References
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Journal ArticleDOI
Engineering of Corneal Tissue through an Aligned PVA/Collagen Composite Nanofibrous Electrospun Scaffold
TL;DR: Human keratocytes and human corneal epithelial cells inoculated on aligned and random PVA-COL electrospun scaffolds adhered and proliferated well, and the aligned nanofibers induced orderly HK growth, indicating that the designed PVA/collagen composite nan ofibrous electroSpun scaffold is suitable for application in tissue-engineered cornea.
Journal ArticleDOI
Cell-biomaterial mechanical interaction in the framework of tissue engineering: insights, computational modeling and perspectives.
TL;DR: A proper understanding of cell-biomaterial interaction, as well as the development of predictive models in this sense, may add some light in tissue engineering and regenerative medicine fields.
Journal ArticleDOI
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death.
Viviana P. Ribeiro,Joana Silva-Correia,Cristiana Gonçalves,Sandra Pina,Hajer Radhouani,Toni Montonen,Jari Hyttinen,Anirban Roy,Ana Oliveira,Rui L. Reis,Joaquim M. Oliveira +10 more
TL;DR: In this article, the authors report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-dimensional (3D) matrix.
Journal ArticleDOI
The addition of SDS to the Bradford dye-binding protein assay, a modification with increased sensitivity to collagen.
TL;DR: The addition of protein to a sub-threshold amount of SDS results in the formation of a green color measurable as an increase in absorbance at 700 nm, in contrast to the blue color measured at 595 nm in the standard assay.
Journal ArticleDOI
Correction to Soft Hyaluronic Gels Promote Cell Spreading, Stress Fibers, Focal Adhesion, and Membrane Tension by Phosphoinositide Signaling, Not Traction Force
Koushik Mandal,Dikla Raz-Ben Aroush,Zachary T. Graber,Bin Wu,Chan Young Park,Jeffery J. Fredberg,Wei Guo,Tobias Baumgart,Paul A. Janmey +8 more
TL;DR: It is suggested that simultaneous signaling stimulated by HA and an integrin ligand can generate phosphoinositide-mediated signals to the cytoskeleton that reproduce those generated by high cellular tension.