Multiplex Polymerase Chain Reaction Assay for Identification of Enterotoxigenic Escherichia Coli Strains
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TLDR
The multiplex PCR system was developed for identification of enterotoxigenic Escherichia coli (ETEC) strains and to differentiate them from other gram negative enteric bacteria and may be used as a method for direct determination of ETEC.Abstract:
A multiplex polymerase chain reaction (PCR) system was developed for identification of enterotoxigenic Escherichia coli (ETEC) strains and to differentiate them from other gram negative enteric bacteria. This test simultaneously amplifies heat-labile (LTI) and heat-stable (STI and STII) toxin sequences and the E. coli-specific universal stress protein (uspA). The specificity of the method was validated by single PCR tests performed with the reference E. coli and non-E. coli strains and with bacteria isolated from pig feces. The multiplex PCR allowed the rapid and specific identification of enterotoxin-positive E. coli and may be used as a method for direct determination of ETEC and to differentiate them from other E. coli and gram-negative enteric isolates.read more
Citations
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Journal ArticleDOI
Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.
Claudia Toma,Yan Lu,Naomi Higa,Noboru Nakasone,Isabel Chinen,Ariela Baschkier,Marta Rivas,Masaaki Iwanaga +7 more
TL;DR: This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube and selected targets selected for each category were eae for enteropathogenic E coli, stx for Shiga toxin-producing E. bacteria, and aggR for enteroaggregative E coli.
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Pathotypes of diarrheagenic Escherichia coli in children attending a tertiary care hospital in South India
Priya Rajendran,Sitara Swarna Rao Ajjampur,Divya Chidambaram,Gunasekaran Chandrabose,Bhuvaneswari Thangaraj,Rajiv Sarkar,Prasanna Samuel,Deva Prasanna Rajan,Gagandeep Kang +8 more
TL;DR: No single assay worked well, but by discounting genes with a pathogenicity index of less than 1, it was possible to use the PCR assays to identify DEC in 75/394 (19%) cases and 12/198 (6.1%) controls, while mixed infection could be identified in 8/394 cases and 2/198 controls.
Journal ArticleDOI
Detection of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 (EAST1) gene and its relationship with fimbrial and enterotoxin markers in E. coli isolates from pigs with diarrhoea.
TL;DR: EAST1 enterotoxin may represent an additional virulence determinant playing a role in the pathogenesis of porcine colibacillosis, as shown in E. coli strains isolated from pigs with postweaning diarrhoea.
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Simultaneous detection of methicillin-resistant Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa by multiplex PCR.
TL;DR: A PCR-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed and resulted in 100% for both positive and negative predictive values.
Book ChapterDOI
Twenty-Five Years of Investigating the Universal Stress Protein: Function, Structure, and Applications.
Amy Cheng Vollmer,Steven J. Bark +1 more
TL;DR: The objective has been to organize the foundational studies in this field to identify the significant impediments to further understanding of Usp functions at the molecular level and propose ideas and experimental approaches that may overcome these impediments and drive future development of molecular approaches to understand and target Usps as central regulators of stress adaptation and survival.
References
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