Journal ArticleDOI
Multipotential differentiation of human urine‐derived stem cells: Potential for therapeutic applications in urology
Shantaram Bharadwaj,Guihua Liu,Yingai Shi,Rongpei Wu,Rongpei Wu,Bin Yang,Bin Yang,Tong-Chuan He,Yuxin Fan,Xinyan Lu,Xiaobo Zhou,Hong Liu,Anthony Atala,Jan Rohozinski,Jan Rohozinski,Yuanyuan Zhang +15 more
TLDR
It is demonstrated that single USCs can expand to a large population with 60–70 population doublings and may be useful in cell‐based therapies and tissue engineering applications, including urogenital reconstruction.Abstract:
We sought to biologically characterize and identify a subpopulation of urine-derived stem cells (USCs) with the capacity for multipotent differentiation. We demonstrated that single USCs can expand to a large population with 60-70 population doublings. Nine of 15 individual USC clones expressed detectable levels of telomerase and have long telomeres. These cells expressed pericyte and mesenchymal stem cell markers. Upon induction with appropriate media in vitro, USCs differentiated into bladder-associated cell types, including functional urothelial and smooth muscle cell lineages. When the differentiated USCs were seeded onto a scaffold and subcutaneously implanted into nude mice, multilayered tissue-like structures formed consisting of urothelium and smooth muscle. Additionally, USCs were able to differentiate into endothelial, osteogenic, chondrogenic, adipogenic, skeletal myogenic, and neurogenic lineages but did not form teratomas during the 1-month study despite telomerase activity. USCs may be useful in cell-based therapies and tissue engineering applications, including urogenital reconstruction.read more
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Exosomal DMBT1 from human urine-derived stem cells facilitates diabetic wound repair by promoting angiogenesis.
Chun-Yuan Chen,Shan-Shan Rao,Lu Ren,Xiong-Ke Hu,Yi-Juan Tan,Yin Hu,Juan Luo,Yi-Wei Liu,Hao Yin,Jie Huang,Jia Cao,Zhen-Xing Wang,Zheng-Zhao Liu,Hao-Ming Liu,Siyuan Tang,Ran Xu,Hui Xie +16 more
TL;DR: The findings suggest that USC-Exos may represent a promising strategy for diabetic soft tissue wound healing by promoting angiogenesis via transferring DMBT1 protein.
Journal ArticleDOI
Exosomes secreted by human urine-derived stem cells could prevent kidney complications from type I diabetes in rats
Zhen-zhen Jiang,Yumei Liu,Xin Niu,Jianyong Yin,Bin Hu,Shang-Chun Guo,Ying Fan,Yang Wang,Nian-song Wang +8 more
TL;DR: USCs-Exo may have the potential to prevent kidney injury from diabetes by inhibiting podocyte apoptosis and promoting vascular regeneration and cell survival.
Journal ArticleDOI
Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering.
TL;DR: Urothelial cells and smooth muscle cells derived from the differentiation of urine-derived stem cells (USC) could be used to form engineered urethral tissue when seeded on a modified 3-D porous small intestinal submucosa (SIS) scaffold.
Journal ArticleDOI
Evaluation of senescence in mesenchymal stem cells isolated from equine bone marrow, adipose tissue, and umbilical cord tissue.
TL;DR: The results demonstrate the limited passage numbers of subcultured BMSCs available for use in research and tissue engineering and suggest that adipose tissue and umbilical cord tissue may be preferable for tissue banking purposes.
Journal ArticleDOI
Tissue Engineering and Regenerative Medicine: Achievements, Future, and Sustainability in Asia.
Fengxuan Han,Jiayuan Wang,Luguang Ding,Yuanbin Hu,Wenquan Li,Zhangqin Yuan,Qianping Guo,Caihong Zhu,Li Yu,Huan Wang,Zhongliang Zhao,Luanluan Jia,Jiaying Li,Yingkang Yu,Weidong Zhang,Genglei Chu,Song Chen,Bin Li +17 more
TL;DR: This review aims to give a brief overview of TERM development in Asia over the last decade by highlighting some of the important advances in this field and featuring major achievements of representative research groups.
References
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Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
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Specific association of human telomerase activity with immortal cells and cancer
Nam Woo Kim,Mieczyslaw A. Piatyszek,Karen R. Prowse,Calvin B. Harley,Michael D. West,Peter L. C. Ho,Gina M. Coviello,Woodring E. Wright,Scott L. Weinrich,Jerry W. Shay +9 more
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Journal ArticleDOI
Identification of c-MYC as a Target of the APC Pathway
Tong-Chuan He,Andrew B. Sparks,Carlo Rago,Heiko Hermeking,Leigh Zawel,Luis T. da Costa,Patrice J. Morin,Bert Vogelstein,Kenneth W. Kinzler +8 more
TL;DR: The c-MYC oncogene is identified as a target gene in this signaling pathway and shown to be repressed by wild-type APC and activated by beta-catenin, and these effects were mediated through Tcf-4 binding sites in the c- MYC promoter.
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Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
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A simplified system for generating recombinant adenoviruses
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