Nature's knockout: the Mel1b receptor is not necessary for reproductive and circadian responses to melatonin in Siberian hamsters.
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TLDR
Data support the hypothesis that the Mel1a receptor, which does encode a functional receptor in this species, mediates reproductive and circadian responses to melatonin.Abstract:
The pineal hormone melatonin regulates seasonal reproduction and influences the timing of circadian rhythms. The Mel1a and Mel1b receptors are the high-affinity melatonin receptors present in mammals. Unexpectedly, the Mel1b receptor gene of the Siberian hamster, Phodopus sungorus, cannot encode a functional receptor; two nonsense mutations are present within the coding region. Southern blot analysis indicates that this is a single copy gene. The Mel1b receptor gene is nonfunctional in outbred populations of P. sungorus and Phodopus campbelli. Siberian hamsters lacking a functional Mel1b receptor nevertheless show seasonal reproductive and circadian responses to melatonin, indicating that the Mel1b receptor is not necessary for these responses. These data support the hypothesis that the Mel1a receptor, which does encode a functional receptor in this species, mediates reproductive and circadian responses to melatonin.read more
Citations
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Melatonin—A pleiotropic, orchestrating regulator molecule
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International Union of Basic and Clinical Pharmacology. LXXV. Nomenclature, Classification, and Pharmacology of G Protein-Coupled Melatonin Receptors
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TL;DR: The steps taken since melatonin's discovery by Aaron Lerner in 1958 are summarized to functionally characterize, clone, and localize receptors in mammalian tissues, as well as current efforts to discover and develop ligands for treatment of a number of illnesses.
References
More filters
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Ligand: a versatile computerized approach for characterization of ligand-binding systems.
Peter J. Munson,David Rodbard +1 more
TL;DR: This approach provides two major advantages compared with other available methods: it uses an exact mathematical model of the ligand-binding system, thereby avoiding the possible biases introduced by several commonly used approximations and it uses a statistically valid, appropriately weighted least-squares curve-fitting algorithm with objective measurement of goodness of fit.
Journal ArticleDOI
Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses
TL;DR: The cloning and characterization of a high affinity receptor for melatonin from the sheep and human is reported, likely mediates the reproductive and circadian actions of melatonin in mammals.
Journal ArticleDOI
Molecular characterization of a second melatonin receptor expressed in human retina and brain: the Mel1b melatonin receptor
Steven M. Reppert,Catherine Godson,Cathy D. Mahle,David R. Weaver,Susan A. Slaugenhaupt,James F. Gusella +5 more
TL;DR: The Mel1b melatonin receptor may mediate the reported actions of melatonin in retina and participate in some of the neurobiological effects ofmelatonin in mammals.