Journal ArticleDOI
Neutrophil-mediated epithelial injury during transmigration: role of elastase
Hedy Ginzberg,Vera Cherapanov,Qin Dong,André M. Cantin,Christopher A. McCulloch,Patrick Shannon,Gregory P. Downey +6 more
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TLDR
Spatially delimited disruption of epithelial junctional complexes with focal loss of E-cadherin, beta-catenin, and zonula occludens 1 was observed adjacent to clusters of transmigrating neutrophils.Abstract:
Neutrophil-mediated injury to gut epithelium may lead to disruption of the epithelial barrier function with consequent organ dysfunction, but the mechanisms of this are incompletely characterized. Because the epithelial apical junctional complex, comprised of tight and adherens junctions, is responsible in part for this barrier function, we investigated the effects of neutrophil transmigration on these structures. Using a colonic epithelial cell line, we observed that neutrophils migrating across cell monolayers formed clusters that were associated with focal epithelial cell loss and the creation of circular defects within the monolayer. The loss of epithelial cells was partly attributable to neutrophil-derived proteases, likely elastase, because it was prevented by elastase inhibitors. Spatially delimited disruption of epithelial junctional complexes with focal loss of E-cadherin, beta-catenin, and zonula occludens 1 was observed adjacent to clusters of transmigrating neutrophils. During neutrophil transmigration, fragments of E-cadherin were released into the apical supernatant, and inhibitors of neutrophil elastase prevented this proteolytic degradation. Addition of purified leukocyte elastase also resulted in release of E-cadherin fragments, but only after opening of tight junctions. Taken together, these data demonstrate that neutrophil-derived proteases can mediate spatially delimited disruption of epithelial apical junctions during transmigration. These processes may contribute to epithelial loss and disruption of epithelial barrier function in inflammatory diseases.read more
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Contribution of neutrophils to acute lung injury.
Jochen Grommes,Oliver Soehnlein +1 more
TL;DR: This review focuses on the mechanisms of neutrophil recruitment into the lung and on the contribution of Neutrophils to tissue damage in ALI.
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Acute Lung Injury: Epidemiology, Pathogenesis, and Treatment
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Severity of E. coli mastitis is mainly determined by cow factors
TL;DR: This review is a compilation of some major findings over the last 15 years concerning mainly host factors that modulate and influence neutrophil function and the mammary inflammatory reaction.
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The role of neutrophils during intestinal inflammation.
TL;DR: The beneficial and deleterious roles of neutrophils in the intestine during health and disease are summarized and an overview of what is known about neutrophil function in the gut is provided.
References
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Journal Article
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