New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples.
TLDR
New molecular-based approaches for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR) to detect mutations to the Cyp51A protein, have been developed which are able to detect mostTriazole-resistant A. fumigatus strains in patients with invasive aspergillosis.Abstract:
The incidence of invasive aspergillosis has increased substantially over the past few decades, accompanied by a change in susceptibility patterns of Aspergillus fumigatus with increasing resistance observed against triazole antifungals, including voriconazole and isavuconazole, the most commonly used antifungal agents for the disease. Culture-based methods for determining triazole resistance are still the gold standard but are time consuming and lack sensitivity. We sought to provide an update on non-culture-based methods for detecting resistance patterns to Aspergillus. New molecular-based approaches for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR) to detect mutations to the Cyp51A protein, have been developed which are able to detect most triazole-resistant A. fumigatus strains in patients with invasive aspergillosis. Over the last few years, a number of non-culture-based methods for molecular detection of Aspergillus triazole resistance have been developed that may overcome some of the limitations of culture. These molecular methods are therefore of high epidemiological and clinical relevance, mainly in immunocompromised patients with hematological malignancies, where culture has particularly limited sensitivity. These assays are now able to detect most triazole-resistant Aspergillus fumigatus strains. Given that resistance rates vary, clinical utility for these assays still depends on regional resistance patterns.read more
Citations
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Journal ArticleDOI
Aspergillus fumigatus and aspergillosis: From basics to clinics.
Amir Arastehfar,Agostinho Carvalho,Jos Houbraken,Lisa Lombardi,Rocio Garcia-Rubio,Jeffrey D. Jenks,Olga Rivero-Menendez,R. Aljohani,Ilse D. Jacobsen,J. Berman,N. Osherov,Mohammad Taghi Hedayati,Macit Ilkit,Darius Armstrong-James,Toni Gabaldón,J. Meletiadis,M. Kostrzewa,Weihua Pan,Cornelia Lass-Flörl,David S. Perlin,Martin Hoenigl,Martin Hoenigl +21 more
TL;DR: In this article, the authors comprehensively discuss the current clinical challenges caused by A. fumigatus and provide insights on how to address them and provide a continuous quest for novel antifungal drugs.
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Invasive aspergillosis in critically ill patients: Review of definitions and diagnostic approaches.
TL;DR: Invasive aspergillosis (IA) is an increasingly recognised phenomenon in critically ill patients in the intensive care unit, including in patients with severe influenza and severe coronavirus disease 2019 (COVID-19) infection as mentioned in this paper.
Journal ArticleDOI
Detecting Azole-Antifungal Resistance in Aspergillus fumigatus by Pyrosequencing.
TL;DR: Current diagnostic methods are reviewed and the potential of pyrosequencing to aid in a diagnosis complete with a resistance profile to improve clinical outcomes is highlighted.
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Diagnosis of Breakthrough Fungal Infections in the Clinical Mycology Laboratory: An ECMM Consensus Statement
Jeffrey D. Jenks,Jean-Pierre Gangneux,Ilan S. Schwartz,Ana Alastruey-Izquierdo,Katrien Lagrou,George Richard Thompson,Cornelia Lass-Flörl,Martin Hoenigl,Martin Hoenigl +8 more
TL;DR: A need exists for diagnostic tests that are effective, simple, cheap, and rapid to enable the diagnosis of bIFI in patients taking antifungals and support the design of future clinical trials in the field of clinical mycology.
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Fungal Infections Diagnosis – Past, Present and Future
TL;DR: A review of the main steps of the diagnosis for systemic fungal infection, from diagnostic classifications, through methodologies considered as the gold standard, to the molecular methods currently used, and finally mentioning some of the more futuristic approaches is presented in this paper.
References
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Performance of Molecular Approaches for Aspergillus Detection and Azole Resistance Surveillance in Cystic Fibrosis.
Hélène Guegan,Sylviane Chevrier,Chantal Belleguic,E. Deneuville,Florence Robert-Gangneux,Jean-Pierre Gangneux +5 more
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Journal ArticleDOI
Fungal Translocation: A Driving Force Behind the Occurrence of Non-AIDS Events?
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TL;DR: Several new diagnostics for IA (including point-of-care tests) are now available to complement galactomannan testing, and immunoPET/MRI imaging may be a promising approach for diagnosing IA in the near future.
Journal ArticleDOI
Antifungal Susceptibility Testing of Aspergillus spp. by Using a Composite Correlation Index (CCI)-Based Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Method Appears To Not Offer Benefit over Traditional Broth Microdilution Testing.
Melissa R. Gitman,Lisa R. McTaggart,Joanna Spinato,Rahgavi Poopalarajah,Erin Lister,Shahid Husain,Shahid Husain,Julianne V. Kus +7 more
TL;DR: It is demonstrated that MALDI-TOF MS can be used to accurately detect A. fumigatus strains with reduced voriconazole susceptibility and rather than proving to be a rapid and simple method for antifungal susceptibility testing, this particular MS-based method showed no benefit over conventional testing methods.
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