New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples.
TLDR
New molecular-based approaches for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR) to detect mutations to the Cyp51A protein, have been developed which are able to detect mostTriazole-resistant A. fumigatus strains in patients with invasive aspergillosis.Abstract:
The incidence of invasive aspergillosis has increased substantially over the past few decades, accompanied by a change in susceptibility patterns of Aspergillus fumigatus with increasing resistance observed against triazole antifungals, including voriconazole and isavuconazole, the most commonly used antifungal agents for the disease. Culture-based methods for determining triazole resistance are still the gold standard but are time consuming and lack sensitivity. We sought to provide an update on non-culture-based methods for detecting resistance patterns to Aspergillus. New molecular-based approaches for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR) to detect mutations to the Cyp51A protein, have been developed which are able to detect most triazole-resistant A. fumigatus strains in patients with invasive aspergillosis. Over the last few years, a number of non-culture-based methods for molecular detection of Aspergillus triazole resistance have been developed that may overcome some of the limitations of culture. These molecular methods are therefore of high epidemiological and clinical relevance, mainly in immunocompromised patients with hematological malignancies, where culture has particularly limited sensitivity. These assays are now able to detect most triazole-resistant Aspergillus fumigatus strains. Given that resistance rates vary, clinical utility for these assays still depends on regional resistance patterns.read more
Citations
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Journal ArticleDOI
Aspergillus fumigatus and aspergillosis: From basics to clinics.
Amir Arastehfar,Agostinho Carvalho,Jos Houbraken,Lisa Lombardi,Rocio Garcia-Rubio,Jeffrey D. Jenks,Olga Rivero-Menendez,R. Aljohani,Ilse D. Jacobsen,J. Berman,N. Osherov,Mohammad Taghi Hedayati,Macit Ilkit,Darius Armstrong-James,Toni Gabaldón,J. Meletiadis,M. Kostrzewa,Weihua Pan,Cornelia Lass-Flörl,David S. Perlin,Martin Hoenigl,Martin Hoenigl +21 more
TL;DR: In this article, the authors comprehensively discuss the current clinical challenges caused by A. fumigatus and provide insights on how to address them and provide a continuous quest for novel antifungal drugs.
Journal ArticleDOI
Invasive aspergillosis in critically ill patients: Review of definitions and diagnostic approaches.
TL;DR: Invasive aspergillosis (IA) is an increasingly recognised phenomenon in critically ill patients in the intensive care unit, including in patients with severe influenza and severe coronavirus disease 2019 (COVID-19) infection as mentioned in this paper.
Journal ArticleDOI
Detecting Azole-Antifungal Resistance in Aspergillus fumigatus by Pyrosequencing.
TL;DR: Current diagnostic methods are reviewed and the potential of pyrosequencing to aid in a diagnosis complete with a resistance profile to improve clinical outcomes is highlighted.
Journal ArticleDOI
Diagnosis of Breakthrough Fungal Infections in the Clinical Mycology Laboratory: An ECMM Consensus Statement
Jeffrey D. Jenks,Jean-Pierre Gangneux,Ilan S. Schwartz,Ana Alastruey-Izquierdo,Katrien Lagrou,George Richard Thompson,Cornelia Lass-Flörl,Martin Hoenigl,Martin Hoenigl +8 more
TL;DR: A need exists for diagnostic tests that are effective, simple, cheap, and rapid to enable the diagnosis of bIFI in patients taking antifungals and support the design of future clinical trials in the field of clinical mycology.
Journal ArticleDOI
Fungal Infections Diagnosis – Past, Present and Future
TL;DR: A review of the main steps of the diagnosis for systemic fungal infection, from diagnostic classifications, through methodologies considered as the gold standard, to the molecular methods currently used, and finally mentioning some of the more futuristic approaches is presented in this paper.
References
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Sven Heldt,Juergen Prattes,Susanne Eigl,Birgit Spiess,Holger Flick,Jasmin Rabensteiner,Gemma L Johnson,Florian Prüller,Albert Wölfler,Tobias Niedrist,Tobias Boch,Peter Neumeister,Heimo Strohmaier,Robert Krause,Dieter Buchheidt,Martin Hoenigl +15 more
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Jeffrey D. Jenks,Helmut J. F. Salzer,Juergen Prattes,Robert Krause,Dieter Buchheidt,Martin Hoenigl +5 more
TL;DR: Detailed guidelines on mucormycosis are needed to reflect the current evidence and give guidance on the use of isavuconazole, a second-generation broad spectrum triazole with a favorable pharmacokinetic and safety profile and few drug–drug interactions.
Journal ArticleDOI
Epidemiology of invasive aspergillosis and azole resistance in patients with acute leukaemia: the SEPIA Study.
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TL;DR: With only two cases of IA due to azole-resistant A. fumigatus, a change of antifungal treatment practices in Germany does not appear warranted currently.
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Comparison of the Sensititre YeastOne colorimetric antifungal panel and Etest with the NCCLS M38-A method to determine the activity of amphotericin B and itraconazole against clinical isolates of Aspergillus spp.
Estrella Martín-Mazuelos,Javier Pemán,Anastasio Valverde,Mónica Chaves,M. Carmen Serrano,Emilia Cantón +5 more
TL;DR: The colorimetric method appears to be a suitable alternative procedure for antifungal susceptibility testing of Aspergillus spp.
Journal ArticleDOI
Azole-resistance in Aspergillus terreus and related species: An emerging problem or a rare Phenomenon?
Tamara Zoran,Bettina Sartori,Laura Sappl,Maria Aigner,Ferran Sánchez-Reus,Antonio Rezusta,Anuradha Chowdhary,Saad J. Taj-Aldeen,Maiken Cavling Arendrup,Salvatore Massimo Oliveri,Dimitrios P. Kontoyiannis,Ana Alastruey-Izquierdo,Katrien Lagrou,Giuliana Lo Cascio,Jacques F. Meis,Walter Buzina,Claudio Farina,Miranda Drogari-Apiranthitou,Anna Grancini,Anna M. Tortorano,Birgit Willinger,Axel Hamprecht,Elizabeth M. Johnson,Lena Klingspor,Valentina Arsic-Arsenijevic,Oliver A. Cornely,Joseph Meletiadis,Wolfgang Prammer,Vivian Tullio,Jorg-Janne Vehreschild,Laura Trovato,Russell E. Lewis,Esther Segal,Peter-Michael Rath,Petr Hamal,Manuel Rodríguez-Iglesias,Emmanuel Roilides,Sevtap Arikan-Akdagli,Arunaloke Chakrabarti,Arnaldo Lopes Colombo,Mariana Soledad Fernández,M. Teresa Martin-Gomez,Hamid Badali,Georgios Petrikkos,Nikolai Klimko,Sebastian M. Heimann,Ömrüm Uzun,Maryam Roudbary,Sonia de la Fuente,Jos Houbraken,Brigitte Risslegger,Cornelia Lass-Flörl,Michaela Lackner +52 more
TL;DR: In this article, a global set of Aspergillus terreus and phenotypically related isolates was molecularly identified (beta-tubulin), tested for antifungal susceptibility against posaconazole, voriconazole and itraconazoles, and resistant phenotypes were correlated with point mutations in the cyp51A gene.
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