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New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae).

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TLDR
These loci will enable genetic diversity studies of P. alba and P. chilensis and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.
Abstract
Premise of the study: As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for Prosopis species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for Prosopis species from a 454 pyrosequencing run were optimized and characterized for studies in P. alba and P. chilensis. Methods and Results: Twelve markers that were successfully amplified showed polymorphism in P. alba and P. chilensis. The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in P. ruscifolia, P. flexuosa, P. kuntzei, P. glandulosa, and P. pallida. Conclusions: These loci will enable genetic diversity studies of P. alba and P. chilensis and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.

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Journal ArticleDOI

Fine-scale spatial genetic structure analysis in two Argentine populations of Prosopis alba (Mimosoideae) with different levels of ecological disturbance

TL;DR: Compared fine-scale SGS between two populations of Prosopis alba: Fernandez-Forres (FF) and Campo Duran (CD), based on the variation of 12 SSR markers, highly significant genetic differentiation was detected with virtually no admixture between populations.
Journal ArticleDOI

De novo assembly of leaf transcriptome, functional annotation and genomic resources development in Prosopis cineraria, a multipurpose tree of Indian Thar Desert

TL;DR: The transcriptome data set reported in the current study will serve as an important public information platform for comparative and functional genomic as well as gene expression studies in P. cineraria and other closely related species.
Journal ArticleDOI

Implications of mating system and pollen dispersal indices for management and conservation of the semi-arid species Prosopis flexuosa (Leguminosae).

TL;DR: The results suggest that for ex situ conservation the distance that would be enough to avoid full sib sampling among harvested seed-trees is about 20 m, and the interaction with long dispersal big herbivores might be considered as a strategy to promote increased outbreeding reducing the rate of mating between related individuals.
Journal ArticleDOI

Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

TL;DR: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers, and the cross-species transferability was high with high percentages of polymorphic loci.
Journal ArticleDOI

Genetic insights into the globally invasive and taxonomically problematic tree genus Prosopis.

TL;DR: The lack of genetic differentiation between most diploid species in their native ranges supports the notion that hybridization between allopatric species may occur frequently when they are co-introduced into non-native areas.
References
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Book ChapterDOI

Primer3 on the WWW for general users and for biologist programmers.

TL;DR: This chapter assumes acquaintance with the principles and practice of PCR, as outlined in, for example, refs.
Journal ArticleDOI

Estimation of average heterozygosity and genetic distance from a small number of individuals

TL;DR: It is shown that the number of individuals to be used for estimating average heterozygosity can be very small if a large number of loci are studied and the average heter homozygosity is low.
Journal Article

Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

TL;DR: A new basis for the construction of a genetic linkage map of the human genome is described, to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA.
Journal ArticleDOI

msatcommander: detection of microsatellite repeat arrays and automated, locus-specific primer design

TL;DR: msatcommander is a platform‐independent program designed to search for microsatellite arrays, design primers, and tag primers using an automated routine, using single‐sequence or concatenated, fasta‐formatted files.
Journal ArticleDOI

Hybridization and genetic variation of Argentine species of Prosopis

TL;DR: The data on genetic variation concerning the percentage of polymorphic loci ( P) and the expected mean of heterozygotes per locus ( H) shows that arboreal species belonging to sect.
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